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A method for microwave-assisted extraction of aconitum alkaloids

A technology of aconitum spp

Active Publication Date: 2016-05-18
GANSU LONGSHENRONGFA PHARMACEUTICAL INDUSTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, up to now, there is no microwave-assisted extraction of alkaloids from Aconitum aconitum in domestic and foreign public literature.

Method used

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  • A method for microwave-assisted extraction of aconitum alkaloids
  • A method for microwave-assisted extraction of aconitum alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 A method for microwave-assisted extraction of aconitum alkaloids, comprising the following steps:

[0024] (1) Add 3 times the mass of ammonia reagent to 100g of Songpanaconitum crushed to 20 meshes, wet it and place it in a microwave extraction device for 2 hours; then add 50 times the mass of Songpanaconitum at a concentration of 80% ethanol, stirred evenly, and extracted for 10 minutes under the conditions of microwave frequency of 2450MHz, power of 700W, and temperature of 80°C to obtain an extract.

[0025] Among them: ammonia reagent refers to the solution formed by mixing ammonia water and water according to the volume ratio of 2mL: 3mL.

[0026] (2) The extract was subjected to suction filtration under the condition of 0.06MPa to obtain the filtrate A; the filtrate A was concentrated under reduced pressure to no ethanol under the condition of a temperature of 30°C and a pressure of 0.07MPa to obtain the aconitum aconitum alkaloid extract .

[0027]...

Embodiment 2

[0030] Example 2 A method for microwave-assisted extraction of aconitum alkaloids, comprising the following steps:

[0031] ⑴Add 4 times the mass of ammonia reagent to 100g of Songpanaconitum crushed to 30 meshes, wet it and place it in a microwave extraction device for 4 hours; then add Songpanaconitum at a mass concentration of 30 times the mass of 95% ethanol, stirred evenly, and extracted under the conditions of microwave frequency of 2450MHz, power of 900W, and temperature of 60°C for 30 minutes to obtain an extract.

[0032] Among them: ammonia reagent refers to the solution formed by mixing ammonia water and water according to the volume ratio of 2mL: 3mL.

[0033] (2) The extract is subjected to suction filtration under the condition of 0.08MPa to obtain the filtrate A; the filtrate A is concentrated under reduced pressure to no ethanol under the condition of a temperature of 50°C and a pressure of 0.08MPa to obtain the aconitum aconitum alkaloid extract .

[0034]...

Embodiment 3

[0037] Example 3 A method for microwave-assisted extraction of aconitum alkaloids, comprising the following steps:

[0038] (1) Add 3.5 times the mass of ammonia reagent to 100 g of Songpanaconitum medicinal material crushed to 25 meshes, wet it and place it in a microwave extraction device for 3 hours; then add 40 times the mass of Songpanaconitum medicinal material at a concentration of 75% ethanol, stirred evenly, and extracted for 20 minutes under the conditions of microwave frequency of 915MHz, power of 800W, and temperature of 70°C to obtain an extract.

[0039] Among them: ammonia reagent refers to the solution formed by mixing ammonia water and water according to the volume ratio of 2mL: 3mL.

[0040] (2) The extract is subjected to suction filtration under the condition of 0.07MPa to obtain the filtrate A; the filtrate A is concentrated under reduced pressure to no ethanol under the condition of a temperature of 60°C and a pressure of 0.09MPa to obtain the aconitum ...

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Abstract

The present invention relates to a microwave-assisted method for extracting alkaloids from Aconitum aconitum. The method comprises the following steps: (1) adding ammonia reagent to the medicinal material of Aconitum aconitum crushed to 20-30 meshes, moistening it and placing it in a microwave extraction device Place it for 2~4h; then add ethanol, stir evenly, and obtain the extract by microwave extraction; (2) the extract is subjected to suction filtration to obtain filtrate A; the filtrate A is concentrated under reduced pressure to no ethanol to obtain Songpan Aconitum biological Alkali extract; (3) add acidic solution to the alkaloid extract to adjust the pH value to 2~4, filter to remove insoluble matter, and obtain filtrate B; the filtrate B is adjusted to pH 10~12 with ammonia reagent, and then diethyl ether -Extract with chloroform solution, combine the extracts; concentrate the extracts under reduced pressure to a dry paste, and obtain the purified aconitum alkaloid product. The present invention is fast and efficient.

Description

technical field [0001] The invention relates to a method for extracting aconitum aconitum alkaloids, in particular to a method for extracting aconitum aconitum alkaloids assisted by microwaves. Background technique [0002] Songpan aconitum (Aconitunsungpanense Hand-Mazz) is a plant of the genus Aconitum in the family Ranunculaceae. It is also known as Huoyanzi, Manwuyao, Yangjiaoqi, and Tengwuyao. It is mainly distributed in Gansu, Qinghai, Sichuan, Shaanxi, Ningxia, Shanxi and other places. It is used by folks. It is used to treat pain and redness caused by bruises, rheumatoid arthritis and other symptoms; its main chemical components are a variety of alkaloids, which have good analgesic effects, and have no tolerance and physical dependence. Therefore, the development and utilization of Songpan aconitum alkaloids have good practical significance. [0003] At present, there are two extraction methods for the extraction of aconitum alkaloids: conventional solvent extractio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/714A61P29/00
Inventor 张喜民王小芳邓月婷张政任一杰吴秀琴
Owner GANSU LONGSHENRONGFA PHARMACEUTICAL INDUSTRY CO LTD