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Application of serum amyloid protein A1

A serum amyloid and protein technology, applied in the application field of serum amyloid A1, can solve problems such as no literature reports, and achieve the effect of promoting the invasion of lung cancer cells

Active Publication Date: 2014-07-09
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SAA1 is a member of the SAA family, but there is no literature report on the relationship between SAA1 and non-small cell lung cancer metastasis

Method used

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  • Application of serum amyloid protein A1
  • Application of serum amyloid protein A1
  • Application of serum amyloid protein A1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Transwell assay to detect cell invasion and migration ability

[0033] (1) Put the aliquoted Matrigel at 4°C overnight to fully dissolve it into a liquid state.

[0034] (2) Dilute Matrigel gel with serum-free cell culture medium DMEM (dilute according to the mass ratio of 1:6).

[0035] (3) Pre-wet the upper chamber of the Transwell with pre-cooled serum-free DMEM culture medium, and remove it after 5 minutes.

[0036] (4) Add 50 μL of diluted gel to the upper chamber of the 24-well transwell with a pre-cooled pipette tip to ensure that the diluted gel in the upper chamber is uniform and free of air bubbles.

[0037] (5) Incubate the transwell at 37°C for at least 4-5 hours to allow it to solidify.

[0038] (6) Digest the LLC cells from the cell culture dish with trypsin, centrifuge at 800rpm for 2 minutes, resuspend the cells in DMEM containing 1% FBS, and count.

[0039] (7) Add 200 μL to the upper chamber containing 2×10 4 For the cell suspension of LLC, plac...

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Abstract

The invention relates to an application of a serum amyloid protein A1. A kit comprises siRNAs shown in SEQ ID NO:1-4 or a polyclonal antibody of the serum amyloid protein A1 of a sequence shown in SEQIDNO:5. The serum amyloid protein A1 as a target of lung cancer metastasis effectively inhibits SAA1 to promote invasion of lung cancer cells by interfering cell SAA1 through siRNA.

Description

technical field [0001] The invention relates to the application of serum amyloid A1 (serum amyloid A1, SAA1) in the field of metastasis diagnosis of non-small cell lung cancer. Background technique [0002] Lung cancer is one of the most common malignant tumors in humans. Worldwide, the mortality rate of lung cancer is as high as 28% of all people suffering from lung cancer. At the same time, the global incidence of lung cancer is rising at a rate of 5% per year, and it is estimated that by 2020, the mortality rate of lung cancer will reach 50%. At present, lung cancer is generally discovered in the late stage, and the early detection methods for lung cancer are still very scarce. Currently, less than 15% of patients suffering from lung cancer can survive for more than 5 years. In the advanced stage of lung cancer, tumor cells will metastasize to the lymph nodes of the whole body and other distant organs, including liver, kidney, brain and bone, etc. More than 90% of lung ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/395A61P35/00
Inventor 陈琪贲晶晶张艳陈丽莉朱旭冬李飞柏惠李晓宇张瀚文杨青
Owner NANJING MEDICAL UNIV
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