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Biomarker for nasopharyngeal carcinoma metastasis diagnosis and/or prognosis evaluation

A prognostic evaluation and biomarker technology, applied in biochemical equipment and methods, microbial measurement/testing, drug combination, etc., can solve problems such as the role and mechanism of circRNA are not completely clear, and achieve the promotion of epithelial-mesenchymal transition of cells , the effect of promoting proliferation

Active Publication Date: 2020-09-29
JIANGSU CANCER HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the roles and mechanisms of most circRNAs in NPC are not fully understood

Method used

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  • Biomarker for nasopharyngeal carcinoma metastasis diagnosis and/or prognosis evaluation
  • Biomarker for nasopharyngeal carcinoma metastasis diagnosis and/or prognosis evaluation
  • Biomarker for nasopharyngeal carcinoma metastasis diagnosis and/or prognosis evaluation

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Materials and methods

[0044] patient sample

[0045] The 40 samples of nasopharyngeal carcinoma were all from the radiotherapy department of Jiangsu Cancer Hospital, and all pathologically confirmed nasopharyngeal carcinoma. According to the Declaration of Helsinki, each patient signed a written informed consent, which was approved by the Ethical Review Committee of Jiangsu Cancer Hospital. The staging standard for nasopharyngeal carcinoma is the eighth edition of UICC. Collection of clinical data: Inclusion criteria: ①No previous treatment; ②Nasopharyngeal carcinoma diagnosed by pathology; ③No history of other tumors; ④Complete clinical and pathological data; ⑤Aged over 18 years old. Exclusion criteria: ①Patients who have received other treatments before treatment; ②Patients with non-primary nasopharyngeal carcinoma; ③Patients with other major diseases that affect radiotherapy; ④Patients with incomplete clinical data and unable to be followed up.

[0046] cell lin...

Embodiment 2

[0077] Example 2 Regulation of Circ-0046263 on the proliferation, invasion and migration of nasopharyngeal carcinoma cells in vivo

[0078] According to the results of in vitro studies, the effect of circ-0046263 on tumor growth in vivo was further verified. We established a nude mouse xenograft tumor model by inoculating knockdown reagents or cells overexpressing circ-0046263 into the soles of male nude mice. The results showed that knockdown of circ-0046263 significantly inhibited tumor growth with smaller tumor volume, while mice treated with overexpression of circ-0046263 had larger tumor weight and volume ( Figure 20-21 ). After 4-6 weeks of injection, the mice were sacrificed, and the inguinal lymph node metastasis was observed. Compared with the respective controls, the number of mice with lymph node metastasis was less in the si-circ-0046263 group, but increased in the circ-0046263 overexpression group ( Figure 19 ). Total proteins were extracted from tumors, and...

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Abstract

The invention discloses a biomarker for nasopharyngeal carcinoma metastasis diagnosis and / or prognosis evaluation. In the invention, we prove that circ-0046263 is highly expressed in a nasopharyngealcarcinoma cell line compared with a normal nasopharyngeal epithelial cell NP69. Clinical tissue sample detection results show that the expression of circ-0046263 in nasopharyngeal carcinoma tissues isup-regulated and is in positive correlation with stages. In-vitro cell function experiments show that circ-0046263 can promote proliferation, migration and invasion of cells and promote epithelial-mesenchymal transition of the cells. Therefore, circ-0046263 can be used as a biomarker to be applied to preparation of a nasopharyngeal carcinoma metastasis diagnosis and / or prognosis evaluation reagent, a kit or a detection device, and also can be used as a target spot to be applied to screening of medicines for treating nasopharyngeal carcinoma or nasopharyngeal carcinoma metastasis; and a reagent for inhibiting circ-0046263 expression can be used for preparing a medicine for treating nasopharyngeal carcinoma or nasopharyngeal carcinoma metastasis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biomarker for nasopharyngeal carcinoma metastasis diagnosis and / or prognosis assessment. Background technique [0002] The incidence of nasopharyngeal carcinoma (NPC) is typically regional, with a high incidence in Southeast Asia. Different from other head and neck tumors, the pathological type of nasopharyngeal carcinoma is mainly poorly differentiated or undifferentiated carcinoma, which is highly malignant and prone to invasion and metastasis. In recent years, with the application of intensity-modulated radiotherapy, the local control rate of nasopharyngeal carcinoma has been significantly improved, but the rate of distant metastasis remains high, which has become the main cause of clinical treatment failure and death, and is currently hindering the long-term survival of nasopharyngeal carcinoma. The biggest bottleneck for further rate increase. Currently, little is known abou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/178C12Q2600/158Y02A50/30
Inventor 尹丽陈杰何侠马承贤汪建林朱晨静葛宜枝
Owner JIANGSU CANCER HOSPITAL
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