Primer for discriminating anguilla marmorata and anguilla bicolor pacifica fry and method thereof

Abstract

The invention provides a pair of primers which can be named as E-EEL170 used for discriminating anguilla marmorata and anguilla bicolor pacifica fry by using microsatellite markers, and a sequence of the primer is shown as SEQ ID No.1 and 2. While discriminating, a common DNA extraction kit is used for extracting DNA of 2 types of eel fry genome and performing PCR amplification on DNA of 2 types of eel DNA samples by the primer pair, 1% of sepharose gel (EB dyeing) electrophoresis is used for detecting the PCR products, strips with 250bp size are anguilla marmorata eel fry, and strips with 500bp size are anguilla bicolor pacifica eel fry. The primer is capable of discriminating anguilla marmorata and anguilla bicolor pacifica fry by taking a few of tail fin without execution of fry, and has the advantages of high stability, good repeatability, strong practicality and easy operation, and can rapidly and accurately discriminate the anguilla marmorata and anguilla bicolor pacifica fry.

Description

Technical field [0001] The invention belongs to the application of molecular marker technology in the production practice in the field of aquaculture. Specifically, the present invention relates to the application of microsatellite marking technology to identify the seedlings of the flower eel and the Pacific bicolor eel. Background technique [0002] Flower eel (Anguilla marmorata) belongs to Bonefish, Anguilla, Anguidae, Eel genus, commonly known as perch, large eel, king eel, king eel, etc., belonging to the river migratory fish, flower eels are distributed in China and Australia , Japan, Southeast Asia and Africa, and other tropical and subtropical regions, are scattered in Fujian, Guangdong, Guangxi, Hainan and Taiwan. Flower eel is rich in nutrition, protein content is higher than that of the same genus eel, and the fat content is much lower than that of the same genus eel. The amino acid composition is rich and complete. It is also called "freshwater ginseng". It is a val...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of identification primer and method for quickly and accurately identifying the young eel and Pacific two-color eel, so as to overcome the defects that existing morphological identification is highly subjective and cannot effectively identify two kinds of eel seedlings. The invention only needs to cut a small amount of caudal fins without killing eel fry, and has the advantages of high stability, simple operation, good repeatability, strong practicability, rapid and accurate identification, etc.

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