Primer for discriminating anguilla marmorata and anguilla bicolor pacifica fry and method thereof

A technology for two-color eel and flower eel, which is applied in the field of aquaculture, can solve the problems of inability to effectively identify two kinds of eel fry, strong subjectivity, etc., and achieves the effects of low cost, obvious difference and simple operation.

Inactive Publication Date: 2014-07-09
NANJING NORMAL UNIVERSITY
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of identification primer and method for quickly and accurately identifying the young eel and Pacific two-color eel, so as to overcome the defects that existing morphological identification is highly subjective and cannot effectively identify two kinds of eel seedlings. The invention only needs to cut a small amount of caudal fins without killing eel fry, and has the advantages of high stability, simple operation, good repeatability, strong practicability, rapid and accurate identification, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer for discriminating anguilla marmorata and anguilla bicolor pacifica fry and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Genomic DNA extraction and quantification of flower eel and Pacific bicolor eel

[0021] Use Beijing Trans Marine Biological Genomics Kit to quickly extract 10-30mg of tail fins of two elvers. (From the sea area of ​​southern Hainan Island, the body length is 7-10cm, after morphological comprehensive identification, it is determined to be the flower eel eel seedling and the Pacific two-color eel eel seedling each of 10) DNA, the kit extracts the sample DNA compared to the traditional method. DNA has the characteristics of high extraction efficiency, fast speed, and high quality of extracted DAN.

[0022] 2. Screening of microsatellite sites and synthesis of primers

[0023] The genomic DNA of the flower eel eel seedling sample obtained in 1 was submitted to the biological company for sequencing, and the obtained data was used to screen out the microsatellite sites and synthesize their primers.

[0024] 3. Screening of identification primers for flower eel eel seedlings and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a pair of primers which can be named as E-EEL170 used for discriminating anguilla marmorata and anguilla bicolor pacifica fry by using microsatellite markers, and a sequence of the primer is shown as SEQ ID No.1 and 2. While discriminating, a common DNA extraction kit is used for extracting DNA of 2 types of eel fry genome and performing PCR amplification on DNA of 2 types of eel DNA samples by the primer pair, 1% of sepharose gel (EB dyeing) electrophoresis is used for detecting the PCR products, strips with 250bp size are anguilla marmorata eel fry, and strips with 500bp size are anguilla bicolor pacifica eel fry. The primer is capable of discriminating anguilla marmorata and anguilla bicolor pacifica fry by taking a few of tail fin without execution of fry, and has the advantages of high stability, good repeatability, strong practicality and easy operation, and can rapidly and accurately discriminate the anguilla marmorata and anguilla bicolor pacifica fry.

Description

Technical field [0001] The invention belongs to the application of molecular marker technology in the production practice in the field of aquaculture. Specifically, the present invention relates to the application of microsatellite marking technology to identify the seedlings of the flower eel and the Pacific bicolor eel. Background technique [0002] Flower eel (Anguilla marmorata) belongs to Bonefish, Anguilla, Anguidae, Eel genus, commonly known as perch, large eel, king eel, king eel, etc., belonging to the river migratory fish, flower eels are distributed in China and Australia , Japan, Southeast Asia and Africa, and other tropical and subtropical regions, are scattered in Fujian, Guangdong, Guangxi, Hainan and Taiwan. Flower eel is rich in nutrition, protein content is higher than that of the same genus eel, and the fat content is much lower than that of the same genus eel. The amino acid composition is rich and complete. It is also called "freshwater ginseng". It is a val...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2531/113
Inventor 尹绍武王小鲁贾一何李丽许芳胡亚丽
Owner NANJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap