Method for screening specific antibody pair for enzyme-linked immuno sorbent assay (ELISA) aiming at protein drugs
A technology for protein drugs and screening methods, applied in biological testing, material testing products, etc., to achieve the effects of excellent detection limit and detection range, excellent sensitivity and detection range
Inactive Publication Date: 2014-07-16
MAB VENTURE BIOPHARM CO LTD
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Problems solved by technology
[0011] The existing literature has not established the ELISA sandwich method for the quantitative analysis of the pharmacokinetics of biological protein drugs, including the systematic report of the antibody pair screening method. The traditional and conventional ELISA antibody pair screening method cannot meet the needs of complex matrices and the existence of a large number of structures. The need for quantitative detection of target drug proteins under analog conditions
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[0026] In order to understand the technical content of the present invention more clearly, the following examples are given in detail.
[0027] The concrete steps of the inventive method are as follows:
[0028] Step 1: Use conventional ELISA antibody pair screening technology to screen antibody pairs for target protein drugs, including capture antibodies and detection antibodies. The screened antibody pairs can be used for ELISA detection of protein drugs, and have better detection sensitivity and detection range. These capture antibody or detection antibody molecules all meet a specific epitope for protein drugs.
[0029] Step 2: Coat the capture antibody or detection antibody screened in step 1 in a 96-well plate, and then add a protein drug labeled with a chromogenic group, and a certain amount of target molecule that the protein drug acts on. Competitive binding reactions were performed in a well plate. After reacting for a period of time, examine the signal value of th...
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The invention relates to a method for screening a specific antibody pair for enzyme-linked immuno sorbent assay (ELISA) aiming at protein drugs. The method is characterized by comprising the following steps: preliminarily screening an antibody pair which can be used for detecting the protein drugs in an ELISA sandwich method, wherein the antibody pair comprises a capture antibody and a detection antibody; confirming that the capture antibody and the detection antibody can block bonding between the protein drugs and action targets thereof through the first detection; confirming that the capture antibody and the detection antibody are binding epitope aiming at the protein drugs and the action targets thereof through the second detection; and confirming that the ELISA sandwich method established by the capture antibody and the detection antibody pair can be used for sample detection in different matrixes through the third detection. According to the method, the specific antibody pairs for ELISA of pharmacokinetic study of the protein drugs can be comprehensively, systematically and efficiently screened.
Description
technical field [0001] The invention relates to the field of biological product detection, in particular to a screening method for specific antibody pairs for ELISA detection, in particular to a screening method for specific antibody pairs for ELISA detection for protein drugs. Background technique [0002] With the continuous advancement of biotechnology, biomacromolecular protein drugs have gradually become the most active and rapid research focus of drug development and have attracted widespread attention. Biological protein drugs include protein polypeptide analogs or derivatives, fusion proteins, monoclonal antibody drugs, etc., which are produced by genetic engineering through expression systems such as bacteria, yeast, or mammalian cells. In the forefront of the bioprotein drug market are monoclonal antibody drugs, which can specifically bind to therapeutic targets, inhibit tumor cell growth or kill tumor cells, and have much fewer side effects than small molecule dru...
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Login to View More IPC IPC(8): G01N33/68
CPCG01N33/68G01N33/94
Inventor 王少雄吕品
Owner MAB VENTURE BIOPHARM CO LTD