Group of antitumor T lymphocytes and preparation method thereof

A lymphocyte and anti-tumor technology, applied in the field of anti-tumor T lymphocyte group and its preparation, can solve the problem of no reported anti-tumor effect, and achieve the effect of inducing cancer cell apoptosis, consolidating treatment and improving treatment.

Inactive Publication Date: 2014-08-13
SHENZHEN KIVITA INNOVATIVE DRUG INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no mixed lymphocyte populations with antitumor effects have been reported

Method used

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  • Group of antitumor T lymphocytes and preparation method thereof
  • Group of antitumor T lymphocytes and preparation method thereof
  • Group of antitumor T lymphocytes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This embodiment provides a method for isolating an anti-tumor T lymphocyte population from a leukocyte-removing filter, comprising the following steps:

[0031] 1) Obtain a 2-unit (400ml) leukocyte-removing filter from the Shenzhen Blood Center. After the blood bank uses the leukocyte-removing filter to filter 400ml of whole blood (normal blood donation), 99% of the leukocytes remain in the filter.

[0032] 2) Rinse the leukocyte-removing filter with 60 ml of 1×PBS to obtain a mixture of PBS, leukocytes and part of the blood.

[0033] 3) Centrifuge the mixture of PBS, white blood cells and part of the blood at 1500 rpm for 20 minutes to discard the plasma.

[0034] 4) Add 30 ml of 1×PBS to the cell mixture from which the plasma was discarded, and mix carefully to form a mixture.

[0035] 5) Carefully superimpose the mixed solution on the liquid surface of the cell separation solution (purchased from Tianjin Haoyang Biological Products Technology Co., Ltd., product numb...

Embodiment 2

[0040] This embodiment provides a method for isolating an anti-tumor T lymphocyte population from a leukocyte-removing filter, comprising the following steps:

[0041] 1) Obtain a 2-unit (400ml) leukocyte-removing filter from the Shenzhen Blood Center. After the blood bank uses the leukocyte-removing filter to filter 400ml of whole blood (normal blood donation), 99% of the leukocytes remain in the filter.

[0042] 2) Rinse the leukocyte-removing filter with 50 ml of 1×PBS to obtain a mixture of PBS, leukocytes and part of the blood.

[0043] 3) Centrifuge the mixture of PBS, white blood cells and part of the blood at 2000 rpm for 15 minutes to discard the plasma.

[0044] 4) Add 30 ml of 1×PBS to the cell mixture from which the plasma was discarded, and mix carefully to form a mixture.

[0045] 5) Carefully superimpose the mixed solution on the liquid surface of the cell separation solution (purchased from Tianjin Haoyang Biological Products Technology Co., Ltd., product numb...

Embodiment 3

[0050] This embodiment provides a method for isolating an anti-tumor T lymphocyte population from a leukocyte-removing filter, comprising the following steps:

[0051] 1) Obtain a 2-unit (400ml) leukocyte-removing filter from the Shenzhen Blood Center. After the blood bank uses the leukocyte-removing filter to filter 400ml of whole blood (normal blood donation), 99% of the leukocytes remain in the filter.

[0052] 2) Rinse the leukocyte-removing filter with 80 ml of 1×PBS to obtain a mixture of PBS, leukocytes and part of the blood.

[0053] 3) Centrifuge the mixture of PBS, white blood cells and part of the blood at 2000 rpm for 20 minutes to discard the plasma.

[0054] 4) Add 40 ml of 1×PBS to the cell mixture from which the plasma has been discarded, and mix carefully to form a mixture.

[0055] 5) Carefully superimpose the mixed solution on the liquid surface of the cell separation solution (purchased from Tianjin Haoyang Biological Products Technology Co., Ltd., product...

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Abstract

The invention provides a group of antitumor T lymphocytes. The group of antitumor T lymphocytes are used for representing the following five types of lymphocyte membrane molecules to a certain extent: human leukocyte differentiation antigens CD3, human leukocyte differentiation antigens CD4, human leukocyte differentiation antigens CD8, human Alpha Beta T cell receptors and human Gamma Delta T cell receptors, thereby inhibiting the proliferation of breast cancers and hepatoma cell lines, inducing the apoptosis of cancer cells as well as inhibiting the growth of the in-vivo tumors of tumor-bearing mice suffering from the hepatoma and the breast cancers. If the antitumor T lymphocytes provided by the invention are matched with the traditional surgery, chemotherapy and radiation therapy, a small quantity of residues and diffused tumor cells are removed and killed in a biological therapy mode after a large quantity of tumor cells are removed by the conventional therapy method, thereby improving and consolidating the tumor therapy and reducing the tumor relapse.

Description

technical field [0001] The invention relates to a group of anti-tumor T lymphocytes and a preparation method thereof. Background technique [0002] In recent years, more and more attention has been paid to the immunotherapy of tumors. Current tumor immunotherapy mainly includes tumor vaccines, monoclonal antibody technology, cytokine therapy, adoptive cellular immunotherapy, etc. Among them, adoptive cellular immunotherapy refers to the transfer of immune cells with anti-tumor activity to tumor patients, directly killing tumors or stimulating the body's anti-tumor immune effect, so as to achieve the purpose of treating tumors. The main adoptive cells include lymphokine-activated killer cells (LAK), tumor-infiltrating lymphocytes (TIL), cytokine-induced killer cells (CIK), CD3 monoclonal antibody-activated killer cells (anti-CD3MeAbactivatedkiller cells), etc. . Of note is the potential role of γδ T cells in adoptive immunotherapy. γδT cells are a special immune cell type...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783A61K35/14A61P35/00
Inventor 蒋宇扬陈妍
Owner SHENZHEN KIVITA INNOVATIVE DRUG INST
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