A kind of breeding method for creating closed glume pollination rice material

Abstract

The invention relates to a breeding method for preparing a closed glume pollination rice material. The method comprises the following steps: selecting target segments in an exon area of a rice closed glume pollination determining gene DEP2, establishing a plant CRISPR / Cas9 target recombinant carrier, introducing into rice cells and regenerating into seedlings, shearing to cause function deletion mutation of the rice cell DEP2 gene, and sequencing the target segments of the regenerated stain genome to obtain stains which carry two equipotential DEP2 genes and at the same time have function deletion mutation, wherein phenotype identification shows that the regenerated strains are converted into closed glume from opened glume in blooming and pollination. The experiment shows that by adopting the method, the closed glume pollination rice material can be rapidly obtained.

Description

technical field [0001] The invention belongs to the field of rice biotechnology breeding, and in particular relates to a breeding method for rapidly creating clam-pollinated rice materials. Background technique [0002] Gene drift (or gene escape, Geneflow) refers to the spontaneous transfer of a target gene of an organism to nearby wild relatives, resulting in internal changes in nearby wild relatives, which have some advantageous characteristics of the target gene and form new species, so that Structural changes have taken place in the entire ecological environment. According to the different targets of transgenic drift, it can be divided into the escape of exogenous genes from transgenic crops to their non-transgenic crops, wild relatives and weeds of the same species. The pathways of gene drift mainly include pollen drift, seed dispersal (or dispersal) and movement of asexual reproductive organs. Genetic drift generally requires a certain medium, such as wind, insects,...

AI Technical Summary

Problems solved by technology

However, some studies have isolated some rice mutants showing closed glume traits, such as: a single recessive gene d7 determines the phenotype of CL mutants (abnormal glume, which combines the outer glume and the inner glume); Mutation of a single recessive gene ld(t)[lodiculelessspikelet(t)] produces lodicule-deleted mutants with non-open glumes; mutation of erect panicle gene DEP2 in rice can produce strict closed glume pollination traits

[0005] Although the varieties of closed glume materials have broad application prospects in rice transgenic breeding, but the natural genetic mutation resources are limited, and there are certain technical difficulties in the application.

Through the traditional backcross introduction technology, the breeding cycle is long, the cost is high, and other donor genome fragments linked to the mutation site may be introduced, which brings unpredictable risks to the variety selection

Images