A kind of cassava micro shoot tip culture medium and culture method

Abstract

The invention discloses a cassava micro stem tip culture medium and a cassava micro stem tip culture method. The cassava micro stem tip culture medium comprises a cassava micro stem tip initial culture medium and a cassava micro stem tip subculture medium, wherein the cassava micro stem tip initial culture medium comprises an MS basal culture medium, naphthylacetic acid with the concentration of 0.01-0.04mg / L, 6-benzyl amino adenine with the concentration of 0.01-0.04mg / L, gibberellin with the concentration of 0.1-3.0mg / L and cane sugar with the concentration of 20-30g / L; the cassava micro stem tip subculture medium comprises an MS basal culture medium, naphthylacetic acid with the concentration of 0.01-0.03mg / L, 6-benzyl amino adenine with the concentration of 0-0.02mg / L, gibberellin with the concentration of 0-0.05mg / L, cane sugar with the concentration of 20-30g / L and carrageenan with the concentration of 6g / L. The cassava micro stem tip culture method comprises the steps of enabling a cassava micro stem tip to be inoculated onto the initial culture medium, and carrying out initial culture to obtain a cassava seedling; and then, enabling the cassava seedling to be inoculated onto the subculture medium, and carrying out subculture to realize rapid propagation. After the cassava micro stem tip culture method is adopted, a complete plant can be obtained by the initial culture, and rapid propagation can be realized by the subculture; and the method is simple and short in culture period.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a cassava micro shoot tip culture medium and a culture method. Background technique [0002] Cassava is currently an important food crop in the world. It is generally propagated asexually by stems. It can be planted throughout the year and is easy to cultivate. It is widely distributed in tropical and subtropical regions around the world. [0003] In recent years, in order to promote the development of my country's cassava industry and enrich the genetic diversity of my country's cassava germplasm resources, new cassava germplasm has been introduced from international organizations such as CIAT and the Brazilian National Institute of Agriculture and Animal Husbandry and foreign research institutions. At the same time as the introduction of new germplasm resources, it is also easy to bring some diseases and insect pests, especially quarantine objects, into the country. Therefore,...

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Problems solved by technology

In the prior art, cassava tissue culture research mainly adopts shoots and stem segments with lateral buds as explants, and there are few related studies on cassava micro shoot tips as explants. In my country, cassava micro shoot tips are used as explants. Tissue culture has not been reported

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