A kind of pcr plate injection molding method

Abstract

The invention relates to the field of injection molding technologies of PCR (Polymerase Chain Reaction) plates and in particular relates to an injection molding method of a PCR plate. The injection molding method of the PCR plate comprises the following steps: A, preparing a PCR plate frame; B, forming a PCR plate cavity in a molding die, enabling the PCR plate cavity to form a PCR plate frame cavity, a connection plate cavity and a plurality of reaction tube cavities, and putting the prepared PCR plate frame into the PCR plate frame cavity; C, injecting molten plastic raw material into the PCR plate cavity through the connection plate cavity to form the PCR plate, wherein a water opening of the prepared PCR plate is formed in the inner side of a connection plate; water openings are formed in reaction tubes, thus the surface quality and performance of the product are improved; the reaction tubes are smooth and neat in surface, stable in size, excellent in light transmission effect and the reaction conditions of samples in the reaction tubes can be well observed and thus the detection effect of the product is improved; meanwhile, the die is few in plastic inlets, so that the structure of the die is simplified, thus the die is high in production efficiency; the product has few water openings, so that the production cost is low, and the raw material is saved.

Description

Technical field: [0001] The invention relates to the technical field of injection molding of PCR boards, in particular to a method for injection molding of PCR boards. Background technique: [0002] At present, in the process of molecular biology research and medical clinical diagnosis, PCR reaction technology is indispensable. The PCR reaction is the polymerase chain reaction (polymerase chain reaction, PCR), which is a method for rapidly amplifying a specific gene or DNA sequence in vitro, so it is also called the in vitro amplification method of genes. On both sides of the DNA fragment to be amplified and two oligonucleotide primers complementary to the two sides, after several cycles of denaturation, annealing and extension, the DNA is amplified 2 square times. For batch amplification, PCR reaction plates are commonly used in PCR reactions, see attached Figure 1~3 As shown, the PCR reaction plate includes a connection plate 1' and a plurality of reaction tubes 2', and ...

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Problems solved by technology

[0003] When the existing PCR reaction plate is injection molded, generally the PCR reaction plate is inverted and carried out injection molding. The injection molding mold includes an upper mold and a lower mold matched with the upper mold. A PCR plate cavity is formed between the lower mold and the PCR plate cavity has a connecting plate cavity 5' and a plurality of reaction tube cavities 6' inverted on the upper end of the connecting plate cavity 5'. A glue inlet 7' is respectively arranged on the top of each reaction tube cavity 6', and raw materials are injected into the PCR plate cavity through each glue inlet 7' at the same time, but the bottom of each reaction tube of the PCR plate produced in this way will form a There is a nozzle, the surface of the bottom of the reaction tube is not smooth, uneven, and the size is unstable, which affects the penetration effect of light, is not conducive to observing the reaction of the sample in the reaction tube, and affects the detection effect, and the injection molding mold requires more glue inlets. The mold structure is complex, the production efficiency is low, the production cost is high, and raw materials are wasted

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