A porous scaffold for cartilage tissue engineering and its preparation method

Abstract

The invention relates to a porous scaffold for cartilage tissue engineering and a preparation method thereof, belonging to the technical field of biomedical materials. Specifically, it is a three-dimensional porous scaffold composed of carrageenan and chitosan, with a porosity of 80-96% and a pore diameter of 50-500 μm; its preparation method is to prepare a carrageenan solution with distilled water, prepare a chitosan solution with a dilute acid solution, and mix it with After beating separately, blending, stirring, adding sodium carbonate crystal powder, beating again, pouring into a stainless steel mold in a water bath, freeze-drying, then immersing in a dilute acid solution, soaking in distilled water, and finally vacuum-drying to obtain cartilage tissue engineering Porous scaffold. The raw materials selected by the invention have a wide range of sources and are safe, and the product has certain functionality, high hardness, high porosity and no harmful residue, and is more suitable for tissue cell culture or cartilage tissue regeneration; the preparation method is simple and easy, and the industrialization prospect is broad.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a porous scaffold for tissue engineering and a preparation method thereof. Background technique [0002] Carrageenan, also known as carrageenan, is a natural anionic polysaccharide plant colloid extracted from red algae. According to different structure types, it can be divided into κ-type carrageenan, ι-type carrageenan and λ-type carrageenan, etc., and κ-type carrageenan has the best coagulation property. Carrageenan is white to light yellowish brown, glossy on the surface, translucent sheet or powder, odorless and tasteless, sticky and slippery in taste, swells in cold water, can be dissolved in hot water above 60 ℃ to form viscous transparent or Slight milky white free flowing solution, insoluble in organic solvents. Carrageenan is easy to gel and has thermal reversibility. After heating and dissolving, it can form a semi-solid transparent gel when it is cooled...

AI Technical Summary

Problems solved by technology

Compared with the traditional microsphere sintering method, the porosity is low; the solution casting / particle leaching method is easy to cause solvent residue, and the volatile solvent is not environmentally friendly; the thermally induced phase separation / freeze drying method is not accurate enough in controlling porosity and pore size; High-tech methods such as spinning method and supercritical fluid preparation method do have great prospects in the future, but their current cost is too high, and large-scale use is still unrealistic

[0005] The cross-linked complex of carrageenan-chitosan has been confirmed by many scholars, and some have also prepared carrageenan-chitosan pellets, but the cross-linked compound of carrageenan-chitosan as a three-dimensional porous scaffold is still There are many problems, such as the hardness of carrageenan is not strong, the porosity of the gel is not high, and the pore size is difficult to control, etc.

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