Tandem mass spectrometry and mass spectrometry

Abstract

The invention relates to a tandem mass spectrometer and a mass spectrometry method, aiming at improving the precision of structural analysis and identification of compounds by collecting information of many product ions in a short time. For this purpose, an ion trap (5) is arranged between the collision cell (3) and the time-of-flight mass separator (6). During the time period during which precursor ions originating from the same compound are selected using the quadrupole mass filter (2), the collision energy is changed from one to the other. Various product ions generated by dissociation under multi-level collision energy respectively and precursor ions that are not dissociated are temporarily trapped in the ion trap (5), and are captured in the form of packets in the state where these ions are mixed is injected and introduced into the time-of-flight mass separator (6) to be subjected to mass spectrometry. Thereby, in the data processing unit ( 21 ), an MS / MS mass spectrum is generated in which the product ions produced in the various dissociation modes occur under the various CID conditions.

Description

technical field [0001] The invention relates to a tandem mass spectrometer and a mass spectrometer method thereof. The tandem mass spectrometer dissociates ions with a specific mass-to-charge ratio by collision-induced dissociation (CID) or other methods, and dissociates through the dissociation method. Mass spectrometry analysis of the resulting product ions (fragment ions). Background technique [0002] In order to identify substances with larger molecular weights and analyze the structure of the substances, MS / MS analysis (or tandem analysis) is known as a method in mass spectrometry. In MS / MS analysis, an ion with a specific mass-to-charge ratio among a plurality of ions generated from a sample is selected as the precursor ion (ie, the first stage of mass separation). By some methods, the precursor ion is Dissociation, these methods include a method in which precursor ions are exposed to the CID gas, and the various product ions produced by the dissociation are separate...

AI Technical Summary

Problems solved by technology

[0006] However, in the mass spectrometer described in Patent Document 1, only two types of information of a highly split mass spectrum and a less split mass spectrum can be obtained, and the mass spectrometer is not always sufficient for analyzing compounds with complex molecular structures. structure

While it is possible to modify the mass spectrometer described in Reference 1 to obtain more than three MS / MS spectra using different CID conditions, it takes some time to perform mass spectrometry for a range of mass-to-charge ratios, and therefore, in The time required to acquire multiple MS / MS spectra for a compound under different CID conditions is correspondingly longer

[0007] Specifically, when a gas chromatograph (GC) and a liquid chromatograph (LC) are connected to the front stage of the mass spectrometer, and compounds temporarily separated by the chromatograph are analyzed by the mass spectrometer, one of the compounds is introduced into the mass spectrometer's The time width is significantly limited

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