Fluorescent probe and detection method of citrate in human urine

A fluorescent probe and citrate technology, applied in the field of chemistry, can solve the problems of high cost and complicated detection, and achieve the effects of saving detection cost, simple pretreatment and easy operation.

Inactive Publication Date: 2014-09-03
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzymatic methods require protein pretreatment and the use of multiple enzymes
All of the above methods require a series of treatment and urine pretreatment processes, and at the same time require the assistance of large instruments, making the detection complicated and costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A fluorescent probe consisting of CdTe quantum dots modified with thioglycolic acid (TGA) and Eu 3+ Eu in solution 3+ Combine composition.

[0021] The thioglycolic acid modified CdTe quantum dots and Eu 3+ The volume ratio of the solution is 1:4;

[0022] The Eu 3+ Eu in solution 3+ The concentration is 2×10 -5 mol / L;

[0023] The preparation method of the CdTe quantum dots modified by the thioglycolic acid (TGA) is:

[0024] CdTe quantum dots were synthesized in aqueous phase, NaHTe was used as Te source, Cd in the synthesis 2+ : Te 2- : The mol ratio of TGA is determined as 1:0.5:2.4, and concrete steps are as follows:

[0025] Preparation of sodium hydride telluride (NaHTe)

[0026] Sequentially weigh 40mg Te powder, 40mg NaHB 4 Put it into a clean reaction bottle, inject 1ml of double distilled water, seal it, put it in the refrigerator at 4°C for 8 hours, and obtain sodium telluride hydride;

[0027] Preparation and Purification of CdTe Quantum Dots

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Abstract

The invention relates to a fluorescent probe and a detection method of citrate in human urine. The fluorescent probe is formed by combining CdTe quantum dot modified by thioglycollic acid (TGA) and Eu<3+> in a Eu<3+> solution; by detecting the fluorescence intensity of the probe before and after adding the citrate, a linear formula is constructed according to the ratio of the fluorescence intensity of the probe before and after adding the citrate and the concentration of the citrate; the fluorescence intensity of the probe before and after adding the human urine is detected under the same conditions, and content of the citrate in the human urine is calculated according to the linear formula. The fluorescent probe is applied to the detection of the citrate in the human urine and has high stability and selectivity, the pretreatment is simple, the operation is easy, and the detection cost is saved.

Description

technical field [0001] The invention belongs to the technical field of chemistry, and in particular relates to a fluorescent probe and a method for detecting citrate in human urine. Background technique [0002] Citrate (citrate) is one of the inhibitors of urinary stone formation in humans. Citrate hinders stone growth and aggregation through a series of complex physiological processes on the stone surface. Hypocitrauria refers to the symptoms of citrate excretion in urine of less than 1.8 mmol in 24 hours, which is very common in patients with urinary stones. Taking potassium citrate drugs can significantly increase the content of citrate in the human body and increase the pH value of urine, so as to achieve the purpose of preventing and treating stone disease. The general survey of urine citrate in asymptomatic people can judge the urinary system disease. The risk of calculus disease can also be initially screened for the etiology of patients with urinary calculi. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88G01N21/64
Inventor 贡佳佳张萍许贤杰朱昌青
Owner ANHUI NORMAL UNIV
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