Selfheal polysaccharide zinc chelate as well as preparation method and application thereof to anti-cancer drug

A technology of zinc chelate and Prunella vulgaris, applied in the application, food preparation, antitumor drug and other directions, to achieve the effects of good product quality, improved reaction efficiency and good solubility

Inactive Publication Date: 2014-09-10
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the preparation of Prunella vulgaris polysa

Method used

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  • Selfheal polysaccharide zinc chelate as well as preparation method and application thereof to anti-cancer drug
  • Selfheal polysaccharide zinc chelate as well as preparation method and application thereof to anti-cancer drug
  • Selfheal polysaccharide zinc chelate as well as preparation method and application thereof to anti-cancer drug

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Experimental program
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Effect test

Embodiment 1

[0035] (1) Prunella vulgaris raw material pretreatment: After drying the Prunella vulgaris fruit ears, crush them, pass through a 30-mesh sieve, add 95% (v / v) ethanol, the mass ratio of Prunella vulgaris powder and 95% ethanol is 1:3, and the temperature 60°C, reflux for 2 hours, centrifuge to obtain the residue, repeat the above operation twice for the residue, and dry at 45°C for 24 hours to obtain the Prunella vulgaris sample;

[0036] (2) Polysaccharide extraction: add distilled water for hot water extraction, the mass ratio of Prunella vulgaris sample to distilled water is 1:20, the temperature is 65°C, the time is 2h, the extract is centrifuged, refluxed twice, and combined for extraction solution, concentrated under reduced pressure to 1 / 4 of the original volume at 50°C, polysaccharide concentrated solution; in the above-mentioned concentrated solution, add Sevag reagent, the volume ratio of concentrated solution and Sevag reagent (chloroform:n-butanol=3:1) 3:1, shake f...

Embodiment 2

[0044] (1) Prunella vulgaris raw material pretreatment: Prunella vulgaris raw material pretreatment: After the Prunella vulgaris fruit ears are dried, pulverize, pass through a 60 mesh sieve, add 95% (v / v) ethanol, the mass ratio of Prunella vulgaris powder to 95% ethanol The ratio is 1:4, the temperature is 90°C, the reflux time is 6h, the residue is obtained by centrifugation, the residue is repeated 4 times, and dried at 60°C for 36h to obtain the Prunella vulgaris sample;

[0045] (2) Polysaccharide extraction: add distilled water and hot water for extraction, the mass ratio of Prunella vulgaris sample to distilled water is 1:40, the temperature is 100°C, the time is 6h, the extract is separated by centrifugation, refluxed for 5 times, and the extract is combined , concentrated under reduced pressure at 60°C to 1 / 6 of the original volume, the polysaccharide concentrated solution; in the above-mentioned concentrated solution, Sevag reagent was added, and the volume ratio of ...

Embodiment 3

[0050] (1) Prunella vulgaris raw material pretreatment: After the Prunella vulgaris fruit ears are dried, pulverize, pass through a 50-mesh sieve, add 95% (v / v) ethanol, the mass ratio of Prunella vulgaris powder and 95% ethanol is 1:5, and the temperature Centrifuge at 75°C for 6 hours to obtain the residue, repeat the above operation 4 times, and dry at 50°C for 30 hours to obtain the Prunella vulgaris sample;

[0051] (2) Polysaccharide extraction: add distilled water for hot water extraction, the mass ratio of Prunella vulgaris sample to distilled water is 1:30, the temperature is 85°C, the time is 4h, the extract is centrifuged, refluxed for 3 times, and combined for extraction solution, concentrated under reduced pressure at 60°C to 1 / 8 of the original volume, polysaccharide concentrated solution; in the above-mentioned concentrated solution, add Sevag reagent, the volume ratio of concentrated solution and Sevag reagent (chloroform:n-butanol=4:1) 6:1, shake for 60 minute...

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Abstract

The invention discloses a selfheal polysaccharide zinc chelate as well as a preparation method and application thereof to an anti-cancer drug. The method comprises the following steps: crushing selfheal clusters, decolorizing by use of ethanol, adding distilled water, performing reflux extraction, deproteinizing the extracting solution, performing ultrafiltration, and freeze-drying the retained liquid to obtain polysaccharide; preparing a polysaccharide solution of which the concentration is 10 mg/mL to 40 mg/mL, heating to the temperature of 40-60 DEG C, regulating pH by using sodium hydroxide, slowly adding a zinc salt solution into the polysaccharide solution, reacting for 6-24 hours, simultaneously performing an discontinuous homogenization process by using a high-shear homogenizer, regulating the pH by using hydrochloric acid, centrifuging to obtain supernatant liquor, putting the supernatant liquor into a dialysis bag for dialysis, collecting the solution in the dialysis bag, and performing freeze-drying to obtain the selfheal polysaccharide zinc chelate. The product disclosed by the invention is simple and easy to implement in preparation method, has a remarkable anti-proliferation effect on human cancer cells and also causes no cytotoxicity to normal cells of the human body, so the selfheal polysaccharide zinc chelate is an ideal anti-cancer drug or a health care product and also has a zinc replenishment effect.

Description

technical field [0001] The invention relates to Prunella vulgaris polysaccharides, the fields of biopharmaceutical engineering and health food, in particular to a Prunella vulgaris polysaccharide zinc chelate, a preparation method thereof and an application in the preparation of anticancer drugs. Background technique [0002] Prunella vulgaris L., known as "self‐heal" in Europe, common names of sheep sausage, lantern head, iron-colored grass, stick stigma, big head flower, etc. fruit ears. Prunella is a perennial plant widely distributed in Europe and China. In China, it is mainly distributed in Jiangsu, Zhejiang, Anhui and Hubei. It produces 4 species and 3 varieties, one of which is introduced. It is cold in nature, pungent and bitter, enters the liver and gallbladder meridians, and has the effects of clearing liver fire, reducing swelling and pain, clearing heat and detoxifying. Prunella vulgaris is a kind of herb with the same origin of medicine and food. In Chinese fo...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P3/02A23L1/09
Inventor 扶雄李超赵振纲黄强游丽君刘瑞海
Owner SOUTH CHINA UNIV OF TECH
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