Single nucleotide polymorphism (SNP) molecular marker primer for major quantitative trait loci (QTL) of pear fruit seed quantity and application thereof

Abstract

The invention discloses a single nucleotide polymorphism (SNP) molecular marker primer for major quantitative trait loci (QTL) of pear fruit seed quantity and an application thereof. A forward primer sequence of an SNP marker Pyd14_051 of major QTL of the pear fruit seed quantity is shown in SEQ ID NO.1, and a reverse primer sequence is shown in SEQ ID NO.2. The specific marker of the pear fruit seed quantity is identified by using a specific primer on the basis of a high-resolution melting curve. The seed quantity can be subjected to good parting by the specific primer, namely the difference of polymorphism express seed quantity on the QTL qSeeds is detected. The SNP marker for the major QTL of the pear fruit seed quantity provided by the invention can be applied to molecular marker-assisted selection breeding of the pear fruit seed quantity, and the SNP marker has important theoretical and practical significance on acceleration of the genetic improvement process of pear species and improvement of the breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and relates to a SNP molecular marker primer for a main effect QTL site of pear fruit seed number and an application thereof. Background technique [0002] my country is one of the most important origins of Pyrus L. in the world, with rich genetic diversity. The cultivars of pears are widely distributed, and they are cultivated except in Hainan Province and Taiwan Province. Pear fruit has high nutritional value, sweet and juicy, and is deeply loved by consumers. The development of pear fruit is closely related to the number of seeds in the fruit. The reason is that the seed is the center for the production of phytohormones, which can produce a physiologically strong "pool" that is conducive to the competition of young fruits for nutrients, thereby ensuring the normal development of the fruit. . Studies have shown that the number of seeds in the fruit is positively correlated with the ...

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Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pear is still in its infancy. Existing research is mainly aimed at some diseases or growth traits, QTL mapping of quantitative traits of pear seeds and detection of polymorphism expression on QTL loci. Differences in seed quantity The development and application of SNP molecular markers has not been reported

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