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Method for detecting mycobacterium tuberculosis

A technology for the detection of Mycobacterium tuberculosis, which is applied in the field of detection of Mycobacterium tuberculosis, can solve the problems of high technology required for the release test of Mycobacterium tuberculosis gamma interferon, cumbersome steps of microbiological examination methods, and low accuracy of rapid detection methods. The effect of short time, low cost and high accuracy

Inactive Publication Date: 2014-09-10
江崇才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the tuberculin test is suitable for laboratory research, but not for disease diagnosis; tubercle bacillus gamma interferon release test requires high technology and high cost; microbiological examination method has cumbersome steps; rapid detection method is not accurate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] A detection method for Mycobacterium tuberculosis,

[0013] The preparation method process of the detection method of described Mycobacterium tuberculosis is as follows:

[0014] 1) Collect samples by direct smear, pick out the smears that can detect acid-fast bacilli, mark such samples, and wait for testing;

[0015] 2) Infect the phages with the sample to be tested, and kill the uninfected phages outside the Mycobacterium tuberculosis, and obtain the phages infected with the Mycobacterium tuberculosis for use;

[0016] 3) amplify the infected phages by the phage obtained in step 2) by biological amplification method, and set aside;

[0017] 4) The phage obtained in step 3) is labeled with gold with an anti-phage antibody, and a rapid immunochromatography method is used to detect the presence or absence of the phage so as to determine whether there is Mycobacterium tuberculosis in the sample.

experiment example

[0019] Experimental objects: 200 patients with different degrees of Mycobacterium tuberculosis infection.

[0020] Experimental method: 200 patients were all detected with the detection method of Mycobacterium tuberculosis of the present invention.

[0021] Experimental results: through the detection method of Mycobacterium tuberculosis of the present invention, all patients have successfully detected Type C Mycobacterium tuberculosis.

[0022] It can be seen from the experimental results that the detection method using the Mycobacterium tuberculosis has high accuracy, low cost, simple steps and short detection time.

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Abstract

The invention discloses a method for detecting mycobacterium tuberculosis. The method comprises the following steps: collecting samples, selecting a sample to be detected, and infecting the sample to be detected by bacteriophages; killing uninfected bacteriophages out of the mycobacterium tuberculosis, and amplifying the infected bacteriophages by a bacteriophage biological amplification method; marking the bacteriophages with gold by anti-bacteriophage anti-bodies, and judging the existence of the mycobacterium tuberculosis in the sample by detecting the existence of the bacteriophages by a rapid immunochromatographic method. The method for detecting mycobacterium tuberculosis has the advantages of high accuracy, low cost, simple steps and short detection time.

Description

technical field [0001] The invention relates to a detection method for Mycobacterium tuberculosis. Background technique [0002] Mycobacterium tuberculosis is the pathogenic bacteria that causes tuberculosis. It can invade all organs of the body, but tuberculosis is the most common. Tuberculosis is still an important infectious disease. An estimated one-third of the world's population is infected with Mycobacterium tuberculosis. According to the WHO, about 8 million new cases occur each year and at least 3 million people die from the disease. Before the founding of the People's Republic of my country, the mortality rate reached 200-300 people / 100,000, ranking first among the causes of death from various diseases. Morbidity and mortality are greatly reduced. However, it should be noted that in some areas of the world, the incidence rate is on the rise again due to AIDS, drug abuse, application of immunosuppressants, alcoholism and poverty. Mycobacterium tuberculosis is a...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56911G01N2800/12
Inventor 江崇才李作臻仇彩霞
Owner 江崇才