Transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate
A technology of palmitic acid triglyceride and palmitic acid, which is applied in the field of oil processing, can solve the problems of unfavorable environmental protection, cumbersome, solvent waste process, etc., and achieve the effect of convenient local materials and simple reaction process
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[0035] Example 1 Separation and purification of fatty acids
[0036] 1. Extraction of fatty acids in lard
[0037] Take 1 g lard, 0.23 g KOH, 2.64 mL 95% ethanol and 0.44 mL water, and react in a water bath at 60° C. for 1 h, then add distilled water and n-hexane to obtain an aqueous phase after extraction. Adjust the pH to 1.0 with hydrochloric acid, and then extract with n-hexane. The obtained oil phase is blown with nitrogen to remove the solvent to obtain a mixed fatty acid.
[0038] 2. Separation of fatty acids
[0039] Take the mixed fatty acid obtained above and stir with acetone at a ratio of 40 mL per gram at 40° C. until all the fatty acids are dissolved. The solution was allowed to cool to room temperature and then stored at -24°C for 24h. After filtering at -24°C, the main component of the obtained solid-phase saturated fatty acid is palmitic acid, which is used as one of the raw materials for the next step of synthesis. The liquid phase obtained by filtration is subjec...
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[0042] Example 2 Synthesis of palmitic acid triglyceride (PPP)
[0043] The lipase from Candida rugosa (lipase from Candida rugosa) was selected as the biocatalyst to catalyze the synthesis of palmitic acid triglyceride from palmitic acid and glycerin separated in Example 1. Take glycerol, the separated palmitic acid and lipase to react in a 1.5 mL centrifuge tube. The reaction is required to be carried out at 60°C without solvent, the reaction time is 4h, the ratio of glycerol to palmitic acid is 1:4, and the amount of enzyme added is 8% of the reaction substrate. The obtained product, palmitate triglyceride, is used as the substrate for the next step of OPO synthesis. After the reaction, the product was dissolved in acetone, the enzyme and unreacted solids were removed by centrifugation, and the supernatant was diluted. After the reaction, 0.1 g of the sample was dissolved in 1 mL of n-hexane for thin-plate chromatography. Scrape the triglyceride strips and dissolve them in ...
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[0045] Example 3 Synthesis of 1,3-dioleic acid-2-palmitate triglyceride
[0046] Take the PPP synthesized in Example 2 and the oleic acid separated and purified in Example 1 into a 1.5 mL centrifuge tube, and add sn-1,3 specific lipase. Under solvent-free conditions, place in a 60℃ water bath for 4h. During the reaction, samples were taken regularly for liquid phase detection. After the acidolysis reaction is completed, the lipase in the reaction product is removed by centrifugal separation to obtain a mixture of triglycerides and fatty acids. After separating triglycerides and free fatty acids by thin-layer chromatography, scrape the glyceride bands and dissolve them in 1 mL of n-hexane for liquid phase detection. The liquid phase detection pattern is shown in figure 2 . The results showed that the reaction temperature was 60°C, the reaction time was 4h, the substrate molar ratio (OA / PPP) was 4:1, and the amount of enzyme added was 7% of the substrate mass. 2-palmitic acid t...
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