Transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate

A technology of palmitic acid triglyceride and palmitic acid, which is applied in the field of oil processing, can solve the problems of unfavorable environmental protection, cumbersome, solvent waste process, etc., and achieve the effect of convenient local materials and simple reaction process

Active Publication Date: 2014-09-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the purity of OPO in the synthetic product is relatively high, the reaction steps are increased, and 2-monoglyceryl palmitate needs to be purified to reach a higher concentration in order to ensure the purit

Method used

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  • Transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate
  • Transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate
  • Transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate

Examples

Experimental program
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Example Embodiment

[0035] Example 1 Separation and purification of fatty acids

[0036] 1. Extraction of fatty acids in lard

[0037] Take 1 g lard, 0.23 g KOH, 2.64 mL 95% ethanol and 0.44 mL water, and react in a water bath at 60° C. for 1 h, then add distilled water and n-hexane to obtain an aqueous phase after extraction. Adjust the pH to 1.0 with hydrochloric acid, and then extract with n-hexane. The obtained oil phase is blown with nitrogen to remove the solvent to obtain a mixed fatty acid.

[0038] 2. Separation of fatty acids

[0039] Take the mixed fatty acid obtained above and stir with acetone at a ratio of 40 mL per gram at 40° C. until all the fatty acids are dissolved. The solution was allowed to cool to room temperature and then stored at -24°C for 24h. After filtering at -24°C, the main component of the obtained solid-phase saturated fatty acid is palmitic acid, which is used as one of the raw materials for the next step of synthesis. The liquid phase obtained by filtration is subjec...

Example Embodiment

[0042] Example 2 Synthesis of palmitic acid triglyceride (PPP)

[0043] The lipase from Candida rugosa (lipase from Candida rugosa) was selected as the biocatalyst to catalyze the synthesis of palmitic acid triglyceride from palmitic acid and glycerin separated in Example 1. Take glycerol, the separated palmitic acid and lipase to react in a 1.5 mL centrifuge tube. The reaction is required to be carried out at 60°C without solvent, the reaction time is 4h, the ratio of glycerol to palmitic acid is 1:4, and the amount of enzyme added is 8% of the reaction substrate. The obtained product, palmitate triglyceride, is used as the substrate for the next step of OPO synthesis. After the reaction, the product was dissolved in acetone, the enzyme and unreacted solids were removed by centrifugation, and the supernatant was diluted. After the reaction, 0.1 g of the sample was dissolved in 1 mL of n-hexane for thin-plate chromatography. Scrape the triglyceride strips and dissolve them in ...

Example Embodiment

[0045] Example 3 Synthesis of 1,3-dioleic acid-2-palmitate triglyceride

[0046] Take the PPP synthesized in Example 2 and the oleic acid separated and purified in Example 1 into a 1.5 mL centrifuge tube, and add sn-1,3 specific lipase. Under solvent-free conditions, place in a 60℃ water bath for 4h. During the reaction, samples were taken regularly for liquid phase detection. After the acidolysis reaction is completed, the lipase in the reaction product is removed by centrifugal separation to obtain a mixture of triglycerides and fatty acids. After separating triglycerides and free fatty acids by thin-layer chromatography, scrape the glyceride bands and dissolve them in 1 mL of n-hexane for liquid phase detection. The liquid phase detection pattern is shown in figure 2 . The results showed that the reaction temperature was 60°C, the reaction time was 4h, the substrate molar ratio (OA / PPP) was 4:1, and the amount of enzyme added was 7% of the substrate mass. 2-palmitic acid t...

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Abstract

The invention discloses a transesterification preparation method for 1,3-dioleic acid-2-triglyceride palmitate, and belongs to the technical field of oil processing. The transesterification preparation method is as follows: separating and extracting to obtain palmitic acid and oleic acid by taking lard oil as a material; synthesizing triglyceride palmitate by utilizing candida rugosa lipase to catalyze palmitic acid and glycerol; and synthesizing 1,3-dioleic acid-2-triglyceride palmitate by utilizing immobilized rhizopus oryzae sn-1,3 specific lipase to catalyze triglyceride palmitate and oleic acid. According to the method provided by the invention, used materials are wide in source and low in price, a reaction process is simple, and product content is higher, and purity can reach 76.19%, and therefore, the transesterification preparation method is suitable for industrial popularization and application.

Description

technical field [0001] The invention relates to an enzymatic transesterification preparation method of 1,3-dioleic acid-2-palmitic acid triglyceride, which belongs to the technical field of oil processing. technical background [0002] Human milk contains about 4%-4.5% fat, 98% of which is triglyceride. Human milk fat provides about 50% energy for infants, and at the same time, it can also provide infants with some essential fatty acids and long-chain polyunsaturated fatty acids, such as DHA, EPA, etc., which can promote the absorption of fat-soluble vitamins and other functions. [0003] Human milk contains triglycerides with special fatty acid composition and structure, which can make the baby absorb minerals and fat better. Not only is this very important for the growth and development of the baby, but it also significantly reduces the likelihood of the baby being constipated. Natural human milk fat has its own unique structural characteristics. In vegetable oil and mil...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 李春刘丽波李艾黎刘宁赵晨王晨旭
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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