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Anti-CD138 monoclonal antibody variable region sequence and preparation method thereof

A monoclonal antibody and variable region technology, applied in the field of biomedicine, can solve problems such as the unclear function of the cytoplasmic region

Inactive Publication Date: 2014-09-24
百奇生物科技(苏州)有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The function of the cytoplasmic domain is still unclear

Method used

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  • Anti-CD138 monoclonal antibody variable region sequence and preparation method thereof
  • Anti-CD138 monoclonal antibody variable region sequence and preparation method thereof
  • Anti-CD138 monoclonal antibody variable region sequence and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] Step 1: Preparation of hybridoma cells

[0053] (1) Animal immunity

[0054] Immunize three 6-8 week-old female healthy BALB / c mice with myeloma tumor cells or CD138 protein antigen respectively, inject 50-100ug antigen / mouse at multiple points in the muscles of both legs, shoulders subcutaneously and intraperitoneally, after 3 times of immunization The antibody titer in the serum was measured by blood collection on 7 days, and the blood was collected once a week. The ELISA detection OD value was greater than 1.0 when the serum was diluted 1:4000, and the BALB / c mice with positive serum WB detection were used for fusion. 3 days before fusion, Intraperitoneal booster injection of antigen without adjuvant, 60ug / monkey.

[0055] (2) Preparation of hybridoma cells

[0056] (2.1) Collection of B lymphocytes

[0057] Three days after the booster immunization, the mice were bled from the eyeballs, and the serum after centrifugation was kept as a positive control. Take out ...

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PUM

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Abstract

The invention provides sixteen nucleotide sequences of an anti-CD138 monoclonal antibody variable region sequence and a preparation method of the anti-CD138 monoclonal antibody variable region sequence. In addition, a sequencing process is carried out. The anti-CD138 monoclonal antibody variable region sequence can be used for preparing recombinant antibodies, ScFv antibodies, humanized antibodies, chimeric antibodies, dual-specific antibodies and single-domain antibodies; can be used for preparing antibody drugs; has a great potential value in aspects of tumor diagnosis, illness state progress, metastatic potential, prognostic evaluation and the like; is an important improvement of development of oncogene in the technical field of biological medicine; and enables a more comprehensive knowledge of values in prediction, diagnosis, treatment and prognosis of cancer to be obtained in the industrial field.

Description

technical field [0001] The invention relates to biomedicine, in particular to an anti-CD138 monoclonal antibody variable region sequence and a preparation method thereof. Background technique [0002] CD138 is a proteoglycan that regulates cellular behavior by interacting with various effectors such as heparin-binding growth factor and soluble matrix components. There are four members of the Syndecans family, Syndecan-1, -2, -3, -4, among which Syndecan-I (CD138) is the most widely studied. [0003] Sydecan-1 (CD138) molecule is a member of the transmembrane syndecan family, which consists of a cytoplasmic segment, a transmembrane segment and an extracellular segment. CD138 is a very complex macromolecular complex with a molecular weight of about 85-92kD. It is a type of sugar conjugate formed by the covalent combination of core protein and unbranched glycosaminoglycan chains, also known as proteoglycan, proteoglycan Sugar exists in all mammals and is an important componen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/30C12N15/13C12N15/63A61K39/395A61P35/00
Inventor 邹建炫杨林张明陈丹杨春花李静文李顺玲吴纯周延庆孙其玲汪伟
Owner 百奇生物科技(苏州)有限公司