RNA interference vector of bactrocera dorsalis cytochrome P450 genes and construction method and application thereof

Abstract

The invention provides an RNA interference vector of bactrocera dorsalis cytochrome P450 genes and a construction method and application of the RNA interference vector and belongs to the technical field of gene engineering. The method comprises the steps that a vector PFGC5941 is used as a frame, and positive and negative specificity target fragments of the bactrocera dorsalis cytochrome P450 genes are inserted in the two sides of an intron of the vector PFGC5941 through double digestion to form an important region of the RNA interference vector; after the constructed RNA interference expression vector is transferred to a plant, the RNA interference vector is transcribed in a somatic cell of the plant to form a double-stranded RNA hairpin structure under the drive of a strong promoter CaMV35S of the RNA interference vector, so that normal expression and translation of a homologous gene fragment in the body of bactrocera dorsalis which eats the plant are influenced. Consequently, normal growth and development of insects can be interfered, and the purpose that the plant resists the bactrocera dorsalis is achieved; the method plays an important role in controlling the bactrocera dorsalis, and the use amount of chemical pesticides is reduced.

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and relates to an RNA interference carrier, in particular to an RNA interference carrier of Bactrocera dorsalis cytochrome P450 gene and its construction method and application. Background technique

[0002] Bactroceradorsalis, also known as Bactroceradorsalis orientalis, is a dangerous quarantine pest in the world. It is widely distributed in major citrus producing areas in China. It poses a serious threat to China's citrus industry and causes huge economic losses to citrus every year. It can harm more than 250 kinds of fruits and vegetables. Bacteralis dorsalis has a wide range of hosts and can harm more than 250 kinds of fruits, vegetables and crops such as citrus, mango and carambola. With the change of climate, the adjustment of planting structure and the increase of international trade, its harm area gradually expands, which brings serious economic losses to the fruit and vegeta...

Images