Paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and application thereof

A technology of Paenoid polymyxa and pathogenic bacteria, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of reduced soil micro-ecological activity, poor growth of cultivated peonies, and reduced ornamental properties, and achieve the effect of promoting rooting

Inactive Publication Date: 2014-10-01
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For ornamental peonies, a prominent problem is that the decrease in soil micro-ecological activity due to the differences in climate environment and soil properties in different regions is one of the important reasons for the poor growth of cultivated peonies and the decline in ornamental properties in the introduced areas.

Method used

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  • Paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and application thereof
  • Paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and application thereof
  • Paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1. Isolation and identification of Paenibacillus polymyxa CSM1101

[0085] 1. Isolation of Paenibacillus polymyxa CSM1101

[0086]Soil samples were collected from the rhizosphere of peony (Paeonia ostii, Fengdan) (6-year-old) plants in Jinshan Village, Jinshan Village, Shun'an Town, Tongling City, Anhui Province, and the soil samples were placed in sterile water and sterilized In a small Erlenmeyer flask with glass beads, shake at 200rpm / min for 20min; take the shaken suspension for serial gradient dilution, 10 3 、10 4 Pipette 200μl of each dilution suspension and spread evenly Nitrogen-free medium, cultured at 30°C for 4 days, picked a single colony, and placed in Nitrogen-free medium solid medium plates were purified by streaking. One of the isolated and purified strains was named CSM1101.

[0087] 2. Identification of nitrogen-fixing bacteria CSM1101 in the rhizosphere of tree peony

[0088] 1. Observation of morphological characteristics and determin...

Embodiment 2

[0115] Example 2. Biological activity of Paenibacillus polymyxa CSM1101

[0116] 1. Determination of Nitrogen Fixation Activity of Paenibacillus polymyxa CSM1101

[0117] Nitrogen fixation activity was determined by acetylene reduction method. Add 2.5mL of semi-solid to a 15mm×15mm screw-top test tube Nitrogen-free medium was inoculated with Paenibacillus polymyxa (Paenibacillus polymyxa) CSM1101CGMCC No.8527, plugged with a cotton plug, and incubated at 30°C for 24 hours; then replaced with a sterile rubber stopper, pumped out 10% of the gas, and injected the same volume of acetylene, Incubate at 30°C for 24h. The gas samples were taken to measure ARA (acetylene reduction activity, acetylene reduction activity) on a Varian VISTA6000 gas chromatograph. The experiment was repeated three times, and the mean value was taken and the variance was not greater than 5%.

[0118] The assay results showed that CSM1101 was in Nitrogen-fixing activity was higher in nitrogen-free me...

Embodiment 3

[0130] Example 3, using corn stalk enzymatic hydrolysis solution to produce bacterial agent A that promotes the growth of peony and antagonizes pathogenic bacteria

[0131] 1. Preparation of fermentation medium

[0132]Take 8000g of corn stalks (calculated by dry weight), cut them into small pieces, put them into a hydrothermal reactor, carry out hydrothermal treatment at 180°C and 1.0Mpa for 20 minutes, and then dry them naturally to obtain pretreated corn stalks; After the pretreated corn stalks were crushed to 100 mesh, cellulase was added at a ratio of 15 FPU per gram of dry straw, and distilled water was added at a ratio of 1L of distilled water per 200 grams of dry straw, at 55°C. 100rpm (radius of rotation: 20mm), hydrolysis at pH=4.8 for 48 hours, cooling and centrifugation, and collecting the supernatant and precipitate respectively. The supernatant is the enzymatic hydrolysis solution, and the precipitate is the enzymatic hydrolysis residue. The glucose content in t...

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Abstract

The invention discloses paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and an application of paenibacillus polymyxa. The paenibacillus polymyxa for resisting multiple peony pathogenic bacteria is paenibacillus polymyxa CSM1101, and the preservation number of paenibacillus polymyxa in the China General Microbiological Culture Collection Center is CGMCC No.8527. The paenibacillus polymyxa CSM1101 can remarkably inhibit Botrytis paeoniae, Cladosporium paeoniae and Cercospora varricolor Winter, and can remarkably promote growth of peony.

Description

technical field [0001] The invention relates to a Paenibacillus polymyxa strain antagonizing various pathogenic bacteria of tree peony and its application. Background technique [0002] Peony belongs to the family Paeoniaceae and the genus Paeonia. It has always been one of the most important ornamental and medicinal plants in China, with a history of more than 1,600 years. At present, the main cultivation places of peonies in China are Heze, Luoyang, Beijing, Linxia, ​​Tianpeng, Tongling and so on. In recent years, the oil value of peony has become increasingly prominent, and the development of peony for oil has shown a trend of rapid expansion. For ornamental peonies, a prominent problem is that the decrease in soil micro-ecological activity due to differences in climate and soil properties in different regions is one of the important reasons for the poor growth of cultivated peonies and the decline in ornamental properties in the introduced areas. As for peony for oil, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00A01P21/00C12R1/01
CPCA01C1/00A01G31/00A01N63/00A01N63/10C12N1/20
Inventor 韩继刚王云山胡永红
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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