Narcissus retrovirus nested RT-PCR detection kit and its detection method

A Narcissus degenerated virus, RT-PCR technology, applied in biochemical equipment and methods, methods based on microorganisms, measurement/testing of microorganisms, etc., can solve problems such as nested RT-PCR detection kits for rapid detection of viruses that have not yet been seen , to achieve the effect of high sensitivity, strong specificity and short cycle
CN104120192BActive Publication Date: 2016-03-16INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2016-03-16

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Abstract

The invention relates to a nest type RT-PCR detection kit and a detection method for narcissus degeneration virus, which are specially used for detecting narcissus degeneration virus. The kit comprises an outer-side forward primer, an outer-side reverse primer, an inner-side forward primer, an inner-side reverse primer, an RT Buffer, an RNA enzyme inhibiting factor, a reverse transcriptase, dNTPs, a PCR Buffer, MgCl2, Taq DNA polymerase, a positive control, a negative control and RNase-free ddH2O. The nest type RT-PCR detection kit and the detection method conduct detection to the narcissus degeneration virus by adopting nest type RT-PCR technology, and have the advantages of being strong in specificity, high in sensitivity, simple and convenient to operate, and fast in detection, and are applicable to fast detection, epidemic situation monitoring and early-warning and forecasting on the narcissus degeneration virus in China export and import quarantine and agricultural production, thus being wide in application prospects.
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Description

technical field

[0001] The invention relates to a nested RT-PCR detection kit for daffodil degeneration virus and a detection method thereof, belonging to the technical field of plant quarantine, and is suitable for rapid detection and monitoring of daffodil degeneration virus in entry and exit and agricultural production. Background technique

[0002] Narcissus degeneration virus (NDV) is a member of the Potyviridae family Potyviridae. The full length of the virus genome is about 9,816bp. It is a positive-sense single-stranded RNA without a polyA tail. The typical pinwheel-shaped inclusions were produced in the mesophyll cells of Narcissus. The virus was first discovered on the British multiflora narcissus variety (Narcissustazettac v. Grand Soleild'Or), and the symptoms of chlorotic stripes appeared on the leaves of infected narcissus. The host of NDV experiments is narrow, mainly limited to several plants of Amaryllidaceae (Amaryllidaceae), and the plants that can be ino...

Claims

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