Recombinant pseudorabies virus, construction method and application thereof

A technique for recombining pseudorabies and viruses, which is applied in the direction of antiviral agents, virus antigen components, viruses/bacteriophages, etc., can solve the problems of raising pig raising costs, high production costs, and expensive prices, and achieve strong protection and improve expression efficiency , the effect of high proliferation titer
CN104130982AInactive Publication Date: 2014-11-05GUANGDONG WENS DAHUANONG BIOTECH +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG WENS DAHUANONG BIOTECH
Publication Date
2014-11-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a recombinant pseudorabies virus (PRV), a construction method and application thereof. The recombinant pseudorabies virus takes recombinant PRV / TK<-> / gE<-> / EGFP<+> as the female parent, an expression cassette containing the main immunogenic gene ORF2 of porcine circovirus type 2 is inserted to replace an EGFP gene expression cassette in a gE gene so as to obtain the recombinant pseudorabies virus named as rPRV-ORF2. The recombinant pseudorabies virus provided by the invention is used for recombinant pseudorabies virus vaccines and has safety and effectiveness.
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Description

technical field

[0001] The invention belongs to the technical field of animal virus genetic engineering, and in particular relates to a recombinant pseudorabies virus and its construction method and application. Background technique

[0002] Pseudorabies virus (PRV) belongs to the Herpesvirus (Herpesviridae) alpha herpesvirus subfamily (Alpha-herpesvirinae) varicella virus (Varicello virus) type I (Porcine herpesvirus Type I), can cause severe disease in pigs . Pseudorabies is often an explosive epidemic in pigs, and it is particularly harmful to pregnant sows, often causing stillbirth, abortion, and mummies; gilts and empty sows infected with the virus can cause infertility, anoestrus, return to oestrus, and relapse. With infertility. After boars are infected, the testicles are swollen and atrophied, and they lose their seeding ability. A large number of newborn piglets die after being infected with PRV, the mortality rate can reach 100% within 15 days of age, and the mo...

Claims

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