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A New Large-Channel Electrophoresis Microchip

A technology of microchips and large channels, which is applied in the direction of material analysis, measurement devices, and instruments by electromagnetic means, can solve the problems of low sample load of capillary microfluidic chips, and achieve the effect of solving the excessive Joule heating effect.

Active Publication Date: 2016-08-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems existing in the prior art, the present invention provides a novel large-channel electrophoresis microchip, which overcomes the problem of low sample loading of the capillary microfluidic chip in the prior art

Method used

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  • A New Large-Channel Electrophoresis Microchip
  • A New Large-Channel Electrophoresis Microchip
  • A New Large-Channel Electrophoresis Microchip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] designed as figure 1 The chip shown, where the distance between B and BW is 10.5cm, and the channel diameter is 1mm; A is the injection site; the distance between S and SW is 1.1cm; the stagger distance between A, S and A, SW is 1mm; A and C The distance between them is 6.5cm; the chips are made according to the steps in the technical scheme. Use the "double T-type" channel electrosampling method to analyze organic acid samples:

[0041] Experimental conditions: buffer: 10mM acetic acid-sodium acetate buffer (containing 0.6% HEC) at pH4.0; sample: 1mg / mL benzoic acid, 2mg / mL p-toluenesulfonic acid mixture; effective length / total length=6.5cm / 10.5cm; detection wavelength: 259nm; injection voltage 300V; injection current 300μA; injection time 60s; negative electrode injection; separation voltage 1000V; current 220-240μA; / min. The result is as Figure 6 , where 1 is p-toluenesulfonic acid; 2 is benzoic acid.

Embodiment 2

[0043] designed as figure 1 The chip shown, where the distance between B and BW is 10.5cm, and the channel diameter is 1mm; A is the injection site; the distance between S and SW is 1.1cm; the stagger distance between A, S and A, SW is 1mm; A and C The distance between C and D is 6.5cm; the channel between C and D adopts the method of inserting a capillary, and the effective length of the channel / total length=13.5cm / 27.5cm is obtained; the chip is manufactured according to the steps in the technical scheme (c). Using the "double T-type" channel electrosampling method and the three-electrode transfer mode to analyze organic acid samples:

[0044] Experimental conditions: one-dimensional buffer: 10mM acetic acid-sodium acetate buffer at pH4.0; two-dimensional buffer: 40mM acetic acid-sodium acetate buffer at pH4.5; samples: 1mg / mL benzoic acid, 2mg / mL Mixed solution of toluenesulfonic acid; negative electrode sample injection; 300V, 90s; transfer voltage: point B is -1000V, poi...

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Abstract

The invention discloses a novel large-channel electrophoresis microchip, which is made of PDMS. The chip is provided with a separation channel with a diameter of 0.8-1.2 mm and a total length of 8-30 cm. The two ends of the separation channel are fixed with buffer tanks, and the inside of the separation channel is fixed There is an internal cooling capillary, the two ends of the internal cooling capillary are fixed with hollow fiber membranes, a sampling channel perpendicular to the separation channel is set at a distance of 0.5 to 2 cm from one end of the separation channel, and a sampling channel is set at a distance of 6 to 20 cm from the sampling channel. There is a transfer channel, the transfer channel is perpendicular to the separation channel, and the material is PDMS or quartz capillary. The total length of the chip is 8-30 cm, the width is 2-9 cm, and the chip thickness is 0.2-0.7 cm. Based on the WBE technology, the invention develops a novel large-channel electrophoresis chip with a separation channel size of millimeter level by means of a microfluidic chip. For the first time, the internal cooling module is introduced in the microfluidic chip electrophoresis system, thereby increasing the sample loading to a level equivalent to that of WBE, and at the same time solving the problem of excessive Joule heating effect, and realizing the zero dead volume connection between the large channel and the transfer channel , the whole process of one-dimensional analysis and two-dimensional transfer of samples can be completed through voltage control.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chip electrophoresis analysis, and in particular relates to an electrophoresis microchip. Background technique [0002] Lab-on-a-chip or Miniaturized Total Analysis System (μ-TAS) was first proposed by Manz and Widmer in 1990, and has been continuously developed in the following 20 years. innovation and development. Generally, the core of microchip electrophoresis technology is high-speed capillary electrophoresis separation in channels with a width of 50-100 μm and a depth of 10-20 μm, which has the advantages of fast analysis speed, high efficiency, low cost, easy automation and high-throughput quantification. Research hotspots in the field of analytical chemistry. With the deepening of current biotechnology research, conventional one-dimensional analysis is difficult to meet the peak capacity requirements of complex samples. Therefore, chip electrophoresis has been developing in the dire...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N27/447
Inventor 李优鑫包建民赵璐萌
Owner TIANJIN UNIV
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