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Test-tube cassava induction method

A technology of test-tube potato and cassava, which is applied in the field of agricultural reproduction, can solve the problems of low induction efficiency and long induction period of cassava test-tube potato, and achieve the effect of shortening the induction of test-tube potato, increasing the induction rate, and improving technical services

Active Publication Date: 2014-11-26
广西壮族自治区农业科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is: for the above-mentioned existing problems, provide a kind of induction method of cassava test tube tuber, solve the problems such as low induction efficiency of cassava test tube tuber induction, induction period is long, so that improve cassava test tube tuber induction efficiency

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Experimental program
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Effect test

Embodiment 1

[0012] Cut the sterile cassava tissue-cultured seedlings into single-bud stem segments, and transfer them to medium A for multiplication and subculture. The culture temperature is 27±1°C, the light intensity is 1500 lx, and the light time is 12h / d. cultured under conditions, the culture period is 40d, medium A is a semi-solid medium of MS+0.01mg / L BA+0.01mg / L NAA+30g / L sucrose, the pH value is 5.8; The single-bud stem section of the cassava tissue culture seedlings was transferred to the induction medium B for cultivation, the cultivation temperature was 27±1°C, the light intensity was 500lx, the light time was 12h / d, and the induction culture period was 45d, wherein The induction medium B is a semi-solid medium of MS+0.01mg / L NAA+60g / L sucrose+1.5g / L activated carbon, pH=5.8.

Embodiment 2

[0014] Cut the sterile cassava tissue-cultured seedlings into single-bud stem segments, and transfer them to medium A for multiplication and subculture. The culture temperature is 27±1°C, the light intensity is 1500 lx, and the light time is 12h / d. cultured under conditions, the culture period is 40d, medium A is a semi-solid medium of MS+0.01mg / L BA+0.01mg / L NAA+30g / L sucrose, the pH value is 5.8; The single-bud stems of the cassava tissue culture seedlings were transferred to the induction medium B for cultivation, the cultivation temperature was 27±1°C, the light intensity was 500 lx, the light time was 12h / d, and the induction culture period was 45d. The induction medium B is a semi-solid medium of MS+0.01mg / L NAA+70g / L sucrose+1.5g / L activated carbon, pH=5.8.

Embodiment 3

[0016] Cut the cassava tissue-cultured seedlings into single-bud stem segments, and transfer them to medium A for multiplication and subculture. The culture temperature is 27±1°C, the light intensity is 1500 lx, and the light time is 12h / d. Under culture, the culture period is 40d, wherein medium A is a semi-solid medium of MS+0.01mg / L BA+0.01mg / L NAA+30g / L sucrose, and the pH value is 5.8; The single-bud stem section of cassava tissue culture seedlings was transferred to the induction medium B for cultivation, the cultivation temperature was 27±1°C, the light intensity was 500-1000 lx, the light time was 12h / d, and the induction culture period was 45d. The induction medium B is a semi-solid medium of MS+0.01mg / L NAA+80g / L sucrose+1.5g / L activated carbon, pH=5.8.

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Abstract

The invention discloses a test-tube cassava induction method and belongs to the field of agricultural breeding methods. The method comprises cassava tissue culture seedling subculture and test-tube cassava induction culture. The cassava tissue culture seedling subculture comprises cutting a sterile cassava tissue culture seedling into single-bud stem sections, transferring the single-bud stem sections to a medium A, and carrying out proliferation subculture. The test-tube cassava induction culture comprises transferring the cassava tissue culture seedling single-bud stem sections subjected to proliferation subculture to an induction culture medium B and carrying out culture under the conditions of a culture temperature of 27+ / -1 DEG C, illumination intensity of 500-1000lx, illumination time of 12h / d and an induction culture period of 45-60d, wherein the induction culture medium B is a semisolid medium containing MS, 0.01-0.02mg / L of NAA, 60-80g / L of cane sugar and 1.5-2.0g / L of active carbon and having a pH value of 5.8. The test-tube cassava induction method solves the problem that test-tube cassava induction efficiency is low and test-tube cassava induction time is long, is convenient for test-tube cassava induction efficiency improvement and provides technical service for cassava scientific research.

Description

technical field [0001] The invention relates to the field of agricultural propagation methods, in particular to the field of cassava propagation methods, and in particular to a method for inducing cassava test tube tubers. Background technique [0002] Cassava (Manihot esculenta Crantz), also known as cassava, cassava, cassava, is a plant of the Euphorbiaceae (Euphorbiaceae) cassava genus (Manihot). The three major food crops, edible, feed and industrial, have huge market development space in our country. [0003] Cassava is mainly harvested from underground tubers. It is of great significance to study the occurrence and development of cassava tubers and the law of starch accumulation. Inducing test-tube tubers under tissue culture conditions will help to explore the rules of cassava root development and starch accumulation. Although there are also domestic and foreign explorations on the induction of cassava test tube tubers, such as Medina et al. (2007) research results s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 严华兵曾文丹周慧文谢向誉陆柳英张雅媛赖大欣
Owner 广西壮族自治区农业科学院经济作物研究所