Method for optimizing vitrified cryopreservation effect of agapanthus embryonic calluses

Abstract

The invention discloses a method for optimizing a vitrified cryopreservation effect of agapanthus embryonic calluses. Cymbidium protocorms are processed by a vitrification solution containing carbon nanomaterials to improve the preservation effect. The method specifically comprises the following steps: preculturing; processing of a loading solution; processing of the vitrification solution; and preserving liquid nitrogen, wherein the vitrification solution contains 0.1-0.5g / L graphene quantum dots. According to the method disclosed by the invention, the preservation effect on the agapanthus embryonic calluses is significantly optimized, and the graphene quantum dots are added as an allogenic material to accelerate cryopreservation of plants by vitrification.

Description

technical field

[0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for optimizing the ultralow temperature preservation effect of vitrification of embryogenic callus of Agapanthus lilifolia. Background technique

[0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the m...

Images