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A method for optimizing the cryopreservation effect of vitrification of embryogenic callus of Agapanthus

An embryogenic callus, vitrification ultra-low temperature technology, applied in the direction of plant cells, can solve the problems of insufficient production and scientific research, and achieve the effect of optimizing the preservation effect, changing the formation status, and reducing damage

Active Publication Date: 2015-08-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant research has established a cryopreservation system for embryogenic callus of Agapanthus alba, and the relative survival rate of cells after preservation has reached 56.94%, but it is still not enough to meet the needs of production and scientific research

Method used

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  • A method for optimizing the cryopreservation effect of vitrification of embryogenic callus of Agapanthus
  • A method for optimizing the cryopreservation effect of vitrification of embryogenic callus of Agapanthus

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Experimental program
Comparison scheme
Effect test

Embodiment

[0052] 1) The embryogenic callus of Agapanthus subcultured for 20 days was pre-cultured at 4°C for 2 days at low temperature on MS solid medium containing 0.5 mol / L sucrose;

[0053] 2) Transfer to the loading solution for soaking at room temperature for 60 minutes;

[0054] 3) Transfer to vitrification solution and dehydrate at 0°C for 40 minutes;

[0055] 4) Finally, store in liquid nitrogen at ultra-low temperature.

[0056] After step 3), without removing the vitrification solution, the embryogenic callus of Agapanthus lily soaked in the vitrification solution is directly placed in liquid nitrogen for cryopreservation.

[0057] According to the above steps, the embryogenic callus of Agapanthus was divided into experimental group and control group.

[0058] Among them, the vitrification solution of the experimental group contained graphene quantum dots of 0.1 g / L, 0.3 g / L, and 0.5 g / L.

[0059] Specifically, the vitrification solution of experimental group 1 contained 0....

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Abstract

The invention discloses a method for optimizing a vitrified cryopreservation effect of agapanthus embryonic calluses. Cymbidium protocorms are processed by a vitrification solution containing carbon nanomaterials to improve the preservation effect. The method specifically comprises the following steps: preculturing; processing of a loading solution; processing of the vitrification solution; and preserving liquid nitrogen, wherein the vitrification solution contains 0.1-0.5g / L graphene quantum dots. According to the method disclosed by the invention, the preservation effect on the agapanthus embryonic calluses is significantly optimized, and the graphene quantum dots are added as an allogenic material to accelerate cryopreservation of plants by vitrification.

Description

technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for optimizing the ultralow temperature preservation effect of vitrification of embryogenic callus of Agapanthus lilifolia. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 申晓辉陈冠群任丽张洁张琰张荻
Owner SHANGHAI JIAO TONG UNIV
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