Method for preparing silybum marianum oil with aqueous enzymatic method

Abstract

The invention provides a method for preparing silybum marianum oil with an aqueous enzymatic method. The method solves the problems of low oil yield, many impurities, low nutritive value and the like of the existing pressing method. The method comprises the steps of carrying out heat treatment and ultrasonic treatment on broken raw materials to thoroughly destroy a cellular structure of oil and increase the oil yield, and carrying out enzymolysis on a material with compound enzyme to ensure more thorough enzymolysis and increase the oil yield. The process requires few procedures; the energy consumption is greatly reduced; the enzyme is used for treating the oil to release the oil from cells; the use of an organic solvent is avoided; a step of recovering the organic solvent is removed; equipment investment and environmental pollution are reduced; operational safety and economy of a technology are improved; at the same time, an operating condition is mild; deterioration of the silybum marianum oil at high temperature is avoided; unsaturated fatty acid in the oil is well protected; and micronutrient substances in the raw material and fine quality of a product are maintained to the full extent.

Description

technical field

[0001] The invention relates to a method for preparing milk thistle oil by using an enzymatic method. Background technique

[0002] The oil content of milk thistle seeds is generally around 20%~30%, which mainly contains 20.87%~36.45% oleic acid, 42.11%~58.89% linoleic acid, 0.9%~2.46% linolenic acid, and mainly linoleic acid Mainly, the total unsaturated fatty acids are as high as 80%. Milk thistle oil also contains β-sitosterol, stigmasterol and other sterols, and is rich in vitamin E, which has high edible value and nutritional value. Milk thistle oil also has high medicinal value. Silybum oil is rich in linoleic acid, which can lower cholesterol and prevent arteriosclerosis.

[0003] Studies have shown that more than 99% of milk thistle oil is distributed in milk thistle seed kernels. Since milk thistle oil is mainly extracted by whole seed pressing at home and abroad, the extracted milk thistle oil has low nutritional value and impurities. Many, deep ...

Examples

specific Embodiment approach 1

[0007] Specific embodiment one: a kind of method that water enzymatic method prepares milk thistle oil realizes through the following steps:

[0008] Step 1: Pretreatment of milk thistle seeds: put them in a drying oven for 24 hours before use, then crush them with a grinder, and sieve them to control the particle size between 40 and 60 mesh;

[0009] Step 2: Heat treatment of oil-containing materials: add water to the pulverized sample in a certain proportion, soak at 45°C for 1 hour to fully swell the sample, then put the feed liquid into a constant temperature water bath, treat at 70°C for 20 minutes, and shake continuously;

[0010] Step 3: Ultrasonic treatment: The materials after heat treatment are extracted by ultrasonic synergy, and the conditions are: ultrasonic power is 400-500w, and synergistic treatment is 1-2h;

[0011] Step 4: Enzyme hydrolysis: Adjust the treated sample to the optimum pH of the added enzyme, add a certain amount of enzyme, put it in a shaking wa...

specific Embodiment approach 2

[0013] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the ratio of material to water in Step 2 is 1:4~1:7. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the enzyme added in step 4 is to add alkaline protease first, and then add compound cellulase for enzymolysis after reacting for a period of time. Other steps are the same as in the first embodiment.

technical field

[0001] The invention relates to a method for preparing milk thistle oil by using an enzymatic method. Background technique

[0002] The oil content of milk thistle seeds is generally around 20%~30%, which mainly contains 20.87%~36.45% oleic acid, 42.11%~58.89% linoleic acid, 0.9%~2.46% linolenic acid, and mainly linoleic acid Mainly, the total unsaturated fatty acids are as high as 80%. Milk thistle oil also contains β-sitosterol, stigmasterol and other sterols, and is rich in vitamin E, which has high edible value and nutritional value. Milk thistle oil also has high medicinal value. Silybum oil is rich in linoleic acid, which can lower cholesterol and prevent arteriosclerosis.

[0003] Studies have shown that more than 99% of milk thistle oil is distributed in milk thistle seed kernels. Since milk thistle oil is mainly extracted by whole seed pressing at home and abroad, the extracted milk thistle oil has low nutritional value and impurities. Many, deep ...

Examples

specific Embodiment approach 1

[0007] Specific embodiment one: a kind of method that water enzymatic method prepares milk thistle oil realizes through the following steps:

[0008] Step 1: Pretreatment of milk thistle seeds: put them in a drying oven for 24 hours before use, then crush them with a grinder, and sieve them to control the particle size between 40 and 60 mesh;

[0009] Step 2: Heat treatment of oil-containing materials: add water to the pulverized sample in a certain proportion, soak at 45°C for 1 hour to fully swell the sample, then put the feed liquid into a constant temperature water bath, treat at 70°C for 20 minutes, and shake continuously;

[0010] Step 3: Ultrasonic treatment: The materials after heat treatment are extracted by ultrasonic synergy, and the conditions are: ultrasonic power is 400-500w, and synergistic treatment is 1-2h;

[0011] Step 4: Enzyme hydrolysis: Adjust the treated sample to the optimum pH of the added enzyme, add a certain amount of enzyme, put it in a shaking wa...

specific Embodiment approach 2

[0013] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the ratio of material to water in Step 2 is 1:4~1:7. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the enzyme added in step 4 is to add alkaline protease first, and then add compound cellulase for enzymolysis after reacting for a period of time. Other steps are the same as in the first embodiment.