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Novel method for sensitively cultivating efficient rice intelligent sterile line by using herbicides

A sterile line and herbicide technology, which is applied in the field of cultivation of intelligent sterile lines, can solve problems such as difficulty in color selection, thickness of rice glume and the like

Inactive Publication Date: 2014-12-17
YANGZHOU UNIV
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has great defects in practical application as a rice intelligent CMS screening marker, because the rice husk is thick and color selection is difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Clone the CYP81A6 gene from rice, construct its RNA interference structure, and construct a linked gene with the fertility restoration gene and pollen inactivation gene of the recessive male sterility gene of rice, and then introduce it through the transformation technology mediated by Agrobacterium The corresponding CMS becomes intelligent CMS. Then the intelligent sterile line is self-crossed, and there are two types of individuals in its offspring, one is the same fertile line as the hybrid obtained from the transgene, that is, the intelligent sterile line, and the other is the sterile line without transgenic components. Breeding plants, and into a 1:1 ratio. In the process of using the population to prepare hybrids, it is only necessary to spray sulfonylurea herbicides such as bendazone to remove non-sterile plants and screen out sterile plants. If the seeds of the intelligent sterile line need to be propagated continuously, only the seeds harvested from the intell...

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PUM

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Abstract

The invention provides a novel method for sensitively cultivating an efficient rice intelligent sterile line by using herbicides. The novel method comprises the following steps: (1) constructing a rice CYP81A6 gene RAN interference structure and constructing a linked gene by a fertile restoring gene and a pollen inactivated gene of a rice recessive male nucleus sterile gene; introducing the linked gene into a corresponding sterile line through an agrobacterium tumefaciens-mediated transformation technology to form an intelligent sterile line; and (2) self-crossing the intelligent sterile line, wherein a descendant group comprises two types of individuals, wherein one type is fertile plants which are the same as heterozygotes obtained by transgenosis, namely the intelligent sterile line, and the other type is sterile plants without transgenic ingredients, and the ratio of the two types is 1 to 1; and spraying sulfonylurea herbicides in a process of preparing hybrids by using the group to remove non-sterile plants, so as to screen out the sterile plants.

Description

technical field [0001] The invention relates to a new method for cultivating high-efficiency rice intelligent sterile lines by using sensitivity to herbicides as an accurate and efficient selection marker for intelligent rice sterile lines, and belongs to the field of intelligent sterile line cultivation. Background technique [0002] The breeding of intelligent sterile lines is the product of the combination of modern biotechnology and traditional breeding methods. It not only makes it possible to use different types of rice recessive male sterility genes, but also realizes extensive matching and screening of strong dominant combinations . However, the currently reported methods (such as color sorting) as screening markers for intelligent sterile lines of rice have great defects in practical application, because the rice hulls are thick and color sorting is difficult. [0003] The successful utilization of rice heterosis depends on the discovery and utilization of sterile ...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 于恒秀刘巧泉梁国华程祝宽冯梦诗张昌泉汤述翥张宏根裔传灯龚志云顾铭洪
Owner YANGZHOU UNIV