Vaccine composition, preparation method and application thereof
A technology of vaccine composition and polymer, applied in the field of vaccine composition, can solve the problems of difficult growth in medium and very harsh nutritional conditions
Active Publication Date: 2014-12-31
PU LIKE BIO ENG
View PDF12 Cites 13 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] Mycoplasma hyopneumoniae has very strict requirements on the nutritional conditions of the culture medium, and it is difficult to grow in general culture medium. The prior art lacks an effective adjuvant vaccine that can enhance immunity against both circovirus and mycoplasma
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0047] Embodiment 1 levamisole hydrochloride solution
[0048] Levamisole hydrochloride was dissolved in deionized water to prepare a 100 mg / ml stock solution and filtered through a 0.2 micron filter.
Embodiment 2
[0049] Embodiment 2.DDA solution
[0050] Dimethyldicoctadecylammonium bromide (DDA; Fluka Analytical) was dissolved in ethanol to make a 15 mg / ml stock solution, and the DDA stock solution was filtered through a 0.2 micron filter.
Embodiment 3
[0051] Embodiment 3. Carbomer solution
[0052] Prepare a 500 mg / mL stock solution by dissolving carbomer in deionized water.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a vaccine composition, which comprises an immune amount of a porcine circovirus antigen, an immune amount of a mycoplasma hyopneumoniae antigen, and levamisole or its derivative, and a polymer. The vaccine composition not only can have an immunological enhancement effect on two antigens at the same time, but also has stability, and can be used for dilution of porcine reproductive and respiratory syndrome live vaccines.
Description
technical field [0001] The invention relates to a vaccine composition, in particular to a dual vaccine composition of porcine circovirus and mycoplasma hyopneumoniae. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus that is the main cause of multisystemic wasting syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph node enlargement, especially inguinal lymph node enlargement up to 5 to 10 times; the lungs mainly show diffuse and interstitial pneumonia changes, the texture is as hard as rubber, and the surface generally has a taupe mottled appearance; the kidney There are many changes, white necrotic lesions of different sizes can be seen scattered in the cortex and medulla, which appear wa...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): A61K39/295A61K39/39A61P31/20A61P31/00
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG