A kind of vaccine composition and its preparation method and application
A vaccine composition and antigen technology, applied in the field of vaccine composition, can solve the problems of difficult growth in the medium and very harsh nutritional conditions
Active Publication Date: 2021-07-23
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- Description
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- Application Information
AI Technical Summary
Problems solved by technology
[0004] Mycoplasma hyopneumoniae has very strict requirements on the nutritional conditions of the culture medium, and it is difficult to grow in general culture medium. The prior art lacks an effective adjuvant vaccine that can enhance immunity against both circovirus and mycoplasma
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0047] Embodiment 1 levamisole hydrochloride solution
[0048] Levamisole hydrochloride was dissolved in deionized water to prepare a 100 mg / ml stock solution and filtered through a 0.2 micron filter.
Embodiment 2
[0049] Embodiment 2.DDA solution
[0050] Dimethyldicoctadecylammonium bromide (DDA; Fluka Analytical) was dissolved in ethanol to make a 15 mg / ml stock solution, and the DDA stock solution was filtered through a 0.2 micron filter.
Embodiment 3
[0051] Embodiment 3. Carbomer solution
[0052] Prepare a 500 mg / mL stock solution by dissolving carbomer in deionized water.
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The invention provides a vaccine composition, which comprises porcine circovirus antigen of immune amount, mycoplasma hyopneumoniae antigen of immune amount, and levamisole or its derivatives and polymers. The vaccine composition not only can simultaneously play an immune enhancing effect on two antigens, but also has stability, and can be used for diluting porcine reproduction and respiratory syndrome live vaccine.
Description
technical field [0001] The invention relates to a vaccine composition, in particular to a dual vaccine composition of porcine circovirus and mycoplasma hyopneumoniae. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus that is the main cause of multisystemic wasting syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph node enlargement, especially inguinal lymph node enlargement up to 5 to 10 times; the lungs mainly show diffuse and interstitial pneumonia changes, the texture is as hard as rubber, and the surface generally has a taupe mottled appearance; the kidney There are many changes, white necrotic lesions of different sizes can be seen scattered in the cortex and medulla, which appear wa...
Claims
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IPC IPC(8): A61K39/295A61K39/39A61P31/20A61P31/00
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG