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In-vitro enzyme digestion method for evaluating amino acid content of soybean meal for poultry

An in vitro digestion and amino acid technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as large variation range, high requirements for instruments and test conditions, and production loss, so as to save feed resources and improve accuracy. performance, and the effect of improving utilization efficiency

Active Publication Date: 2015-01-07
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the traditional method for determining the effective amino acids of soybean meal needs to be carried out through metabolic tests, which has problems such as long time required for determination and high requirements for instruments and test conditions. It is basically impossible to measure except for special research, and formula design in feed production can only be done through The calibration value or empirical value of the existing database is carried out
However, the effective amino acid content in soybean meal is significantly related to its processing technology, crude protein content and raw material sources, etc., and the range of variation is large. The formula design is only based on a certain fixed value in production, which can easily lead to excess or insufficient effective amino acid in the formulated feed. , resulting in loss of production

Method used

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  • In-vitro enzyme digestion method for evaluating amino acid content of soybean meal for poultry
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  • In-vitro enzyme digestion method for evaluating amino acid content of soybean meal for poultry

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Embodiment 1

[0033] 1, gather 2 kinds of soybean meal samples, mark respectively as 1# soybean meal and 2# soybean meal; Measure its amino acid content (AA i ) and in vitro dry matter disappearance rate (DMD), the measured results were substituted into the constructed mathematical model to obtain the content of each effective amino acid (see Table 1 and Table 2 for the measurement results).

[0034] In order to verify the coincidence between the assessed value and the real value of effective amino acids in soybean meal poultry, the measured values ​​of effective amino acids in two kinds of soybean meal samples were determined according to the following method: Healthy adult Hailan brown roosters with similar body weight were placed in a single cage in the environment-controlled metabolic testing room. Raise a single animal, the house temperature is 24°C±3°C, the light intensity is 20LX, and the daily light time is 16h. During the non-experimental period, the roosters were fed with a full-p...

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Abstract

The invention relates to an in-vitro enzyme digestion method for evaluating the effective amino acid content of soybean meal for poultry. The amino content (AAi) of a soybean meal sample is analyzed and measured at first, a two-step enzyme (pepsin-pancreatin) method is adopted to digest soybean meal in vitro, then regression analysis is carried out based on the measured values of amino acid content of the soybean meal sample plus in-vitro dry matter digestion rate (AAi*DMD) and the corresponding effective amino acid content for poultry (EAAi), and finally a mathematic model representing the relationships among the contents of various amino acids in the soybean meal sample, the in-vitro dry matter digestion rate, and the effective amino acid content is established and can be used to quickly evaluate the effective amino acid content of soybean meal for poultry. The provided method is capable of rapidly evaluating the effective amino acid content of soybean meal for poultry, is suitable for being applied to production, and provides a reference for more precisely preparing a poultry feed.

Description

(1) Technical field [0001] The invention relates to a method for evaluating the effective amino acid content of soybean meal and poultry by using an in vitro two-step enzyme digestion method, belonging to the technical field of feed. (2) Background technology [0002] Protein raw materials account for about 40-50% of the total cost of livestock and poultry feed. Therefore, accurate evaluation of the nutritional value of protein feed raw materials and improving the utilization efficiency of protein raw materials are important means and methods to reduce the cost of compound feed. Soybean meal is currently the most widely used protein feed material in my country's feed industry, accounting for more than 20% of poultry feed. Effective amino acid is an important index to evaluate the nutritional value and availability of soybean meal. It has better repeatability and applicability than traditional apparent digestible amino acid and total amino acid, and can more accurately reflec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37C12Q1/34
Inventor 林海焦洪超赵梅赵景鹏宋志刚
Owner SHANDONG AGRICULTURAL UNIVERSITY
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