Method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria

A technology of biomarkers and cyanobacteria, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as low tolerance limits the economic feasibility of bioethanol

Inactive Publication Date: 2015-01-07
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In recent years, the gradual replacement of traditional fossil fuels with bioethanol produced by photosynthetic microalgae has attracted in

Method used

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  • Method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria
  • Method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria
  • Method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method for finding biomarkers related to photosynthetic cyanobacteria and ethanol tolerance comprises the following steps:

[0035] (1) carry out ethanol treatment culture to cyanobacteria:

[0036] The cyanobacteria photosynthetic cyanobacterium Synechocystis wild-type was cultured in fresh BG-11 medium under the conditions of light intensity of 50 μmol / m^2-s, rotation speed of 130 rpm, and temperature of 30°C. The cyanobacteria grew to OD630 When it is 0.2, take two 10ml bacterial solutions and centrifuge, one of which is supernatant removed and added to 50ml fresh BG-11 medium as a control group; the other is added to 50ml fresh BG-11 containing ethanol As the experimental group in the culture medium, the volume concentration of ethanol in the experimental group is 1.9%; the above two groups of samples all have three parallel controls, the control group and the experimental group are 50 μmol / m^2-s in the light intensity, and the rotating speed is 130rpm , cultiv...

Embodiment 2

[0057] The method for finding biomarkers related to photosynthetic cyanobacteria and ethanol tolerance comprises the following steps:

[0058] (1) carry out ethanol treatment culture to cyanobacteria:

[0059] The wild-type cyanobacteria photosynthetic cyanobacteria Synechocystis sp. was cultured in fresh BG-11 medium under the conditions of light intensity of 45 μmol / m^2-s, rotation speed of 130 rpm, and temperature of 28°C. The cyanobacteria grew to OD630 When it is 0.2, take two 10ml bacterial solutions and centrifuge, one of which is supernatant removed and added to 50ml fresh BG-11 medium as a control group; the other is added to 50ml fresh BG-11 containing ethanol In the culture medium as the experimental group, the volume concentration of ethanol in the experimental group is 1.0%; the above two groups of samples have three parallel controls, and the light intensity of the control group and the experimental group is 45 μmol / m^2-s, and the rotating speed is 130rpm , cult...

Embodiment 3

[0070] The method for finding biomarkers related to photosynthetic cyanobacteria and ethanol tolerance comprises the following steps:

[0071] (1) carry out ethanol treatment culture to cyanobacteria:

[0072] The cyanobacteria photosynthetic cyanobacterium Synechocystis wild-type was cultured in fresh BG-11 medium under the conditions of light intensity of 40 μmol / m^2-s, rotation speed of 130 rpm, and temperature of 29°C, and the cyanobacteria grew to OD630 When it is 0.2, take two 10ml bacterial solutions and centrifuge, one of which is supernatant removed and added to 50ml fresh BG-11 medium as a control group; the other is added to 50ml fresh BG-11 containing ethanol As the experimental group in the culture medium, the volume concentration of ethanol in the experimental group is 2.0%; the above two groups of samples all have three parallel controls, and the light intensity of the control group and the experimental group is 40 μmol / m^2-s, and the rotating speed is 130rpm ,...

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Abstract

The invention discloses a method for finding biomarkers relevant to tolerance to ethyl alcohol of photosynthetic cyanobacteria. The method comprises the following steps: (1) carrying out ethyl alcohol treatment culture on cyanobacteria; (2) extracting metabolin; (3) performing GC-MS (Gas Chromatography-Mass Spectrometer) detection and data treatment; and (4) carrying out principal component analysis. The method can rapidly find out the biomarkers of the cyanobacteria in an ethyl alcohol stress environment; the biomarkers can provide targets for metabolism path research of ethyl alcohol tolerance of the cyanobacteria, so as to further provide fundamental basis for improving ethyl alcohol tolerance of the cyanobacteria; and the method also can provide a new thinking and a method for optimization of the ethyl alcohol tolerances of other strains.

Description

technical field [0001] The invention belongs to the field of biofuels and relates to a method for searching biomarkers related to photosynthetic cyanobacteria and ethanol tolerance. Background technique [0002] In recent years, the gradual replacement of traditional fossil fuels with bioethanol produced by photosynthetic microalgae has attracted increasing attention. However, the current low tolerance of microalgae to ethanol severely limits the economic feasibility of bioethanol production. Therefore, improving the tolerance of microalgae to ethanol is of great significance for the production of biofuels. [0003] Cyanobacteria are common autotrophic prokaryotes with extremely wide distribution on the earth and strong tolerance to environmental stress. Because of their ability to perform photosynthesis and nitrogen fixation, cyanobacteria grow better in harsh environments than other microorganisms. And cyanobacteria are easy to cultivate on a large scale under laboratory...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N30/06
CPCG01N30/06G01N30/88
Inventor 朱烨石朦亮牛向凤陈磊张卫文
Owner TIANJIN UNIV
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