Rapid breeding method for tissue culture of bambusa texlitis

A technology of tissue culture and green bamboo, which is applied in the field of plant cultivation, can solve the problems such as not easy to harden, and achieve the effect of high rooting rate, low energy consumption and high quality

Inactive Publication Date: 2015-01-21
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bambusa seldom blooms and is not easy to bear fruit, so seeds are rarely used for seedling cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the tender stems of 2 nodes of Bambusa vegetalis, remove the leaves and terminal buds with a length of about 3mm, wash them in 20% bleach solution for 15 minutes, put 9% calcium hypochlorite on the ultra-clean workbench, and add two drops of Tween, Treat for 16 minutes, rinse with sterile water for 5 times, disinfect the stems of green bamboo shoots, insert WPM+ZT3mg / L+NAA0.1mg / L+ Guangdamycin 15mg / L+0.65% agar+30g / L sucrose for culture The buds were induced in the medium, pH5.80, light intensity 2700lx, photoperiod 13h / d, temperature 27℃, and the induced axillary buds were put into WPM+BAP0.3mg / L+Cad1.5mg / L+cysteine ​​100mg / Proliferate clustered buds in L+0.65% agar+30g / L sucrose medium, pH5.80, light 3500lx, photoperiod 15h / d, temperature 27℃, cultured clustered shoots were inserted into LS+NAA0.1mg / L +Cad1mg / L, add 0.65% agar, 50g / L sucrose to induce rooting, pH5.80, light 10000lx, photoperiod 18h / d, temperature 28°C, the rooting rate was calculated after one m...

Embodiment 2

[0011] Take the tender stems of 2 nodes of Bambusa vegetalis, remove the leaves and terminal buds with a length of about 3mm, wash them in 20% bleach solution for 15 minutes, put 9% calcium hypochlorite on the ultra-clean workbench, and add two drops of Tween, Treat for 16 minutes, rinse with sterile water for 5 times, disinfect the stems of green bamboo shoots, insert WPM+ZT3mg / L+NAA0.1mg / L+ Guangdamycin 15mg / L+0.65% agar+30g / L sucrose for culture The buds were induced in the medium, pH5.80, light intensity 2700lx, photoperiod 13h / d, temperature 27°C, the induced axillary buds were put into WPM+BAP0.4mg / L+Cad2mg / L+cysteine ​​100mg / L+ Proliferate clustered buds in 0.65% agar + 30g / L sucrose medium, pH 5.80, light 3500lx, photoperiod 15h / d, temperature 27°C, cultured clustered shoots were inserted into LS+NAA0.1mg / L+Cad1mg / L, add 0.65% agar, 50g / L sucrose to induce rooting, pH 5.80, light 10000lx, photoperiod 18h / d, temperature 28°C, the rooting rate was calculated after one m...

Embodiment 3

[0013] Take the tender stems of 2 nodes of Bambusa vegetalis, remove the leaves and terminal buds with a length of about 3mm, wash them in 20% bleach solution for 15 minutes, put 9% calcium hypochlorite on the ultra-clean workbench, and add two drops of Tween, Treat for 16 minutes, rinse with sterile water for 5 times, disinfect the stems of green bamboo shoots, insert WPM+ZT3mg / L+NAA0.1mg / L+ Guangdamycin 15mg / L+0.65% agar+30g / L sucrose for culture The buds were induced in the medium, pH5.80, light intensity 2700lx, photoperiod 13h / d, temperature 27°C, and the induced axillary buds were put into WPM+BAP0.5mg / L+Cad2mg / L+cysteine ​​100mg / L+ Proliferate clustered buds in 0.65% agar + 30g / L sucrose medium, pH 5.80, light 3500lx, photoperiod 15h / d, temperature 27°C, cultured clustered shoots were inserted into LS+NAA0.1mg / L+Cad1 .2mg / L, add 0.65% agar, 50g / L sucrose to induce rooting, pH 5.80, light 10000lx, photoperiod 18h / d, temperature 28°C, the rooting rate was calculated after...

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PUM

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Abstract

The invention relates to a rapid breeding method for the tissue culture of bambusa texlitis. The method comprises the steps of obtaining sterile explants, inducing axillary buds, carrying out propagation on the axillary buds, and carrying out root induction. According to the method, a rapid breeding technology system for the tissue culture of the bambusa texlitis is built, so that the multiplication coefficient is increased, and the large-area popularization and planting can be conveniently realized in the production process.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of green bamboo, and belongs to the technical field of plant cultivation. Background technique [0002] green bamboo, Bambusa textilis McClure , also known as strip bamboo, mountain green bamboo, yellow bamboo, small green bamboo, grass family, distributed in southern China, including Guangdong, Guangxi, Taiwan, Hunan, Fujian, southern Yunnan, equivalent to the area south of 25 degrees north latitude, within its distribution range The annual average temperature is 18-20°C, and the annual rainfall is above 1400mm. It can grow well, and it can grow well in loose, moist, and fertile soil; it can grow on river banks, plains, hills, and all around (especially on both sides of rivers, basins, and plains. Grows best on alluvial soils). Suitable for growing in warm and humid climate environment. It is mainly produced in Guangdong, with the most in Guangning County, and it is also the lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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