A method for ultra-fine crushing of the cell wall of large seaweed Asparagus

A technology of fine crushing and cell wall, applied in the field of ultrafine crushing of the cell wall of large seaweed asparagus, achieving the effect of low processing cost, easy access to processing equipment, and less labor required

Active Publication Date: 2016-11-30
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the deepening of research, some wall-breaking technologies have also obtained better extraction results, but most of them are limited to the preparation of a small number of samples in the laboratory. Therefore, it is of great significance to find a fast and industrially-operable wall-breaking technology.

Method used

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  • A method for ultra-fine crushing of the cell wall of large seaweed Asparagus
  • A method for ultra-fine crushing of the cell wall of large seaweed Asparagus
  • A method for ultra-fine crushing of the cell wall of large seaweed Asparagus

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Experimental program
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Effect test

Embodiment 1

[0019] (1) Fresh dark reddish-brown thick asparagus-981 (981 asparagus) from Nan'ao Island, Shantou, Guangdong, or asparagus-981 stored at low temperature, washed with water and drained.

[0020] (2) Mix the above-mentioned asparagus with PBS buffer according to the ratio of weight to volume (W (g) / V (ml)), asparagus: PBS buffer = 1:2, mix and put into cylindrical ice Store in the freezer at -20°C for 5 hours.

[0021] (3) Adjust the ultra-fine crushing device so that the dihedral angle formed by the ice-shaving knife and the ice-shaving table is 50°-60°, and the vertical height between the blade of the ice-shaving knife and the ice-shaving table is less than 0.5mm.

[0022] (4) Place the above-mentioned frozen seaweed ice cubes in the above-mentioned adjusted ultra-fine crushing device for crushing.

[0023] (5) Collect the broken seaweed ice in the above step (4), melt the broken seaweed ice at 4°C and then freeze it again at -20°C, then repeat the above breaking, repeat 5 ...

Embodiment 2

[0026] (1) Fresh dark reddish-brown thick asparagus-981 from Nan'ao Island, Shantou, Guangdong Province or asparagus-981 stored at low temperature, washed with water and drained.

[0027] (2) Mix the above-mentioned asparagus with PBS buffer according to the ratio of weight to volume (W (g) / V (ml)), asparagus: PBS buffer = 1:2, mix and put into cylindrical ice Place in the tank at -80°C for 3 hours.

[0028] (3) Adjust the ultra-fine crushing device so that the dihedral angle formed by the ice-shaving knife and the ice-shaving table is 50°-60°, and the vertical height between the blade of the ice-shaving knife and the ice-shaving table is less than 0.5mm.

[0029] (4) Place the above-mentioned frozen seaweed ice cubes in the above-mentioned adjusted ultra-fine crushing device for crushing.

[0030] (5) Collect the broken seaweed ice in the above step (4). The crushed seaweed ice melts at 25°C and freezes again at -80°C, then repeats the above breaking. Repeat 3 times to compl...

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Abstract

The invention relates to cell crushing technology, in particular to an ultrafine crushing method for cell walls of large seaweed asparagus. Mix asparagus and PBS buffer solution according to the weight-to-volume ratio of 2-4:1 (W / V), and freeze-broken for 3 to 5 times after mixing, the complete wall breaking of seaweed can be realized; adopt the present invention Methods The diameter of the algae particles in the seaweed mud obtained after breaking the wall can generally reach 0.1-0.5mm, and the algae mud is diluted ten times. After leaching for 48 hours, the purity of phycoerythrin reaches 0.35, and the dissolution rate reaches 43.91% (mg / g). . The method has the advantages of simple equipment, easy operation, high wall breaking rate, and is beneficial to the dissolution of biologically active substances.

Description

technical field [0001] The invention relates to cell crushing technology, in particular to an ultrafine crushing method for cell walls of large seaweed asparagus. Background technique [0002] The large seaweed Asparagus is rich in phycoerythrin and bromoperoxidase. As the main raw material for extracting phycoerythrin and bromoperoxidase, it is also widely planted in my country. Since the improved new variety "Asparagus-981" has been cultivated on a large scale in Nan'ao, Shantou, Guangdong, asparagus has become the third largest algae cultivation industry after kelp and seaweed. The average yield per mu has reached 3.5 tons, and the national planting area has reached 20. This provides the possibility for large-scale separation and purification of phycoerythrin and bromoperoxidase. [0003] Asparagus algae are upright, linear, cylindrical, clustered, 30-50cm high, up to 1m or more in length. Phycoerythrin and bromoperoxidase in asparagus are intracellular substances. To f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B02C18/00
Inventor 刘冰谷洋洋杜虹秦松麦伟群陈伟洲
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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