Capillary electrophoresis direct ultraviolet method for rapidly detecting Amadori compound based on complexation and online sweeping technique

A technology of capillary electrophoresis and complexation, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of expensive equipment, time-consuming cleaning, time-consuming, etc., and achieves convenient operation, strong reliability, and high performance. The effect of sensitivity
CN104297326AInactive Publication Date: 2015-01-21JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2015-01-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a capillary electrophoresis direct ultraviolet method for rapidly detecting an Amadori compound based on complexation and an online sweeping technique, and relates to the detection of an Amadori compound in foods such as tomato products, dried fruits and tobaccos so as to further guide food processing. An Amadori compound and Cu<2+> are utilized by the method so as to form a stable complex, and the complex has characteristics of ultraviolet absorption and wavelength red shift which are higher than those of the Amadori compound, the capillary electrophoresis separation power is provided by combining the complexing capability of Amadori compound and Cu<2+> and different charge-to-mass ratios; the detection sensitivity and the detection limit are improved further by utilizing the capillary electrophoresis online sweeping technique, and the capillary electrophoresis online sweeping technique can be used for detecting the Amadori compound in tomato powder, and dried fruits such as raisin, and tobaccos. The capillary electrophoresis direct ultraviolet method has the prominent advantages that the Amadori compound in the samples is not required to be subjected to derivatization treatment, the operation is convenient, the Amadori compound can be effectively separated and determined within a short time of 30 minutes, sensitiveness and detection limit are relatively high, efficiency and convenience are realized, and the reliability is high.
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Description

technical field

[0001] Rapid detection of Amadori compounds by capillary electrophoresis direct ultraviolet method based on complexation and online sweeping technology. The invention relates to the detection of Amadori compounds in tomato products, dried fruit products, tobacco and other foods, so as to further guide food processing. Method using Amadori compounds with Cu 2+ Form a stable complex, and the complex has stronger ultraviolet absorption and wavelength red shift characteristics than the Amadori compound itself, combined with the Amadori compound-Cu 2+ Different complexing ability and charge-to-mass ratio provide the driving force for capillary electrophoresis separation, and the online sweeping technology of capillary electrophoresis further improves the detection sensitivity and detection limit, which can be used for the detection of Amadori compounds in tomato products such as tomato powder, dried fruits such as raisins, and tobacco . The invention belongs to t...

Claims

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