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Honeysuckle quality detection method

A quality detection method, the technology of honeysuckle, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of small number of active ingredient peaks, poor separation effect, long detection time, etc., to achieve good repeatability and stability, High detection efficiency and reasonable detection time

Inactive Publication Date: 2015-01-21
NANJIANG BAICAO TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the face of such complex active ingredients, how to ensure the quality of the drug and control its curative effect requires the control of its active substance group. However, the existing detection methods generally have a long detection time and a small number of detected active ingredient peaks. , poor separation effect and other technical problems, therefore, it is particularly important to seek a fast and effective detection method for the quality control of honeysuckle

Method used

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  • Honeysuckle quality detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment one honeysuckle detection method

[0033] 1. Preparation of extract solution for test

[0034] Accurately weigh 0.2g of honeysuckle, put it in a 50ml measuring bottle after pulverization, add 50% methanol to dissolve it, then ultrasonicate for 30min, let it cool down, set the volume to 50ml, and filter to obtain the test solution.

[0035] 2. Liquid chromatography conditions

[0036] 3. Liquid chromatography conditions

[0037] Mobile phase: mobile phase A: acetonitrile; mobile phase B: 0.3% (w / v) citric acid and 2% (w / v) tetrahydrofuran aqueous solution, eluted by gradient, the elution program is:

[0038] 0-3min, mobile phase A: mobile phase B = 95:5

[0039] At 15min, mobile phase A: mobile phase B = 90:10

[0040] At 20min, mobile phase A: mobile phase B = 75:25

[0041] At 40min, mobile phase A: mobile phase B = 60:40

[0042] At 45min, mobile phase A: mobile phase B = 0:100

[0043] Start flushing at 45 minutes and equilibrate the column for 15 m...

Embodiment 2

[0046] Embodiment two different batches of honeysuckle detection method

[0047] Liquid Chromatography Conditions

[0048] Mobile phase: mobile phase A: acetonitrile; mobile phase B: 0.3% (w / v) citric acid and 2% (w / v) tetrahydrofuran aqueous solution, eluted by gradient, the elution program is:

[0049] 0-3min, mobile phase A: mobile phase B = 95:5

[0050] At 15min, mobile phase A: mobile phase B = 90:10

[0051] At 20min, mobile phase A: mobile phase B = 75:25

[0052] At 40min, mobile phase A: mobile phase B = 60:40

[0053] At 45 minutes, mobile phase A: mobile phase B = 0:100.

[0054] Start flushing at 45 minutes and equilibrate the column for 15 minutes.

[0055] Column temperature: 30°C; Flow rate: 1.0ml / min; Detection wavelength: 352nm; Injection volume: 10ul

[0056] Prepare honeysuckle sample solution according to the sample preparation method in embodiment 1, carry out liquid chromatography detection to the sample solution of 10 batches respectively, test re...

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Abstract

The invention provides a honeysuckle quality detection method. The method mainly comprises the following steps: dissolving honeysuckle with 50% methyl alcohol, carrying out ultrasonic treatment, making up to constant volume, and filtering so as to prepare a test solution; and detecting active ingredients in the test solution according to a gradient elution program by taking octadecylsilane chemically bonded silica as filler and taking acetonitrile as a mobile phase A and 0.3% (w / v) citric acid and a 2% (w / v) tetrahydrofuran aqueous solution as a mobile phase B. According to the established honeysuckle detection method, a majority of pharmacological active materials in the honeysuckle are effectively represented, so that the chemical ingredients in the honeysuckle are comprehensively represented, and the one-sidedness that the overall quality of the honeysuckle is judged by only detecting a few of chemical ingredients in the honeysuckle is avoided; the peak separability of all active ingredients in the honeysuckle is good, and the peaks of the main active ingredients in the honeysuckle appear within 35 minutes. The method is reasonable in detection time and good in repeatability and stability.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a method for detecting the quality of honeysuckle. Background technique [0002] Honeysuckle, also known as honeysuckle, is the flower buds and vine leaves of the perennial semi-evergreen entwining woody vine Lonicerae Lonicerae in the Lonicera family. It is a common traditional herbal medicinal material for both medicine and food. It has been planted in my country for more than 2,200 years. The name "Honeysuckle" comes from "Compendium of Materia Medica". Because the honeysuckle flower is white at first, and then turns yellow, it is named honeysuckle. Honeysuckle generally produces four-season flowers, and the flowering period is between May and October. There are differences between the north and the south, and the closer to the south, the earlier the flowering period. Among the four seasons flowers, the first and second seasons are the most obvious, especially the first season, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 余赟
Owner NANJIANG BAICAO TRADITIONAL CHINESE MEDICINE