Application of reduced coenzyme II
A reduced and coenzyme technology, applied in the biological field, can solve problems such as slow onset of action
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[0031] 1 Materials and methods
[0032] 1.1 Sample:
[0033] 1.2 Experimental animals: five-month-old middle-aged male ICR mice; eight-month-old old male C57 mice.
[0034] 1.3 Instruments and reagents: swimming box, lead wire, full-wavelength microplate reader, constant temperature water bath, centrifuge, liver glycogen detection kit (Nanjing Jiancheng, product number A043), lactic acid detection kit (Nanjing Jiancheng, product number A019-1) , Urea nitrogen kit (Nanjing Jiancheng, article number C013-1), SOD detection kit (Nanjing Jiancheng, article number A001-1), MDA detection kit (Nanjing Jiancheng, article number A003-1).
[0035] 1.4 Experimental method:
[0036] A. The first batch: 60 male five-month-old middle-aged ICR mice were randomly divided into 4 groups, 15 in each group. The low-dose group received 2.5 mg / kg intraperitoneal injection, the middle-dose 5 mg / kg intraperitoneal injection, and the high-dose 10 mg / kg intraperitoneal injection. The control group was...
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