Diacetyl spermine detection kit and preparation method and application thereof

A technology of diacetylspermine and detection kits, which is applied in the field of biological detection, can solve the problems of elevated tumor incidence, missed treatment time, etc., and achieve the effect of simple operation

Active Publication Date: 2015-01-28
LIAONING MEDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in a developing country like China, where the level of medicine and sanitation is not as advanced as t...

Method used

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  • Diacetyl spermine detection kit and preparation method and application thereof
  • Diacetyl spermine detection kit and preparation method and application thereof
  • Diacetyl spermine detection kit and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Kit preparation:

[0045] ①Take 1ml of colloidal gold, add 7μL of 0.2M K 2 CO 3 After mixing, add 20 μg DiAcSpm monoclonal antibody, mix and let stand for 10 minutes, add 10 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, and redissolve the precipitate with 1000 μL reconstitution solution;

[0046] ②Take 1ml of colloidal gold, add 2-10μL of 0.2M K 2 CO 3 After mixing, add 10-30 μg CRE monoclonal antibody, mix and let stand for 10 minutes, add 10 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, and redissolve the precipitate with 1000 μL reconstitution solution;

[0047] ③Take 2ml of colloidal gold, add 10μL of 0.2M K 2 CO 3 After mixing, add 30 μg rabbit antibody, mix and let stand for 10 minutes, add 20 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, an...

Embodiment 2

[0053] Kit preparation:

[0054] ①Take 1ml of colloidal gold, add 7μL of 0.2M K 2 CO 3 After mixing, add 20 μg DiAcSpm monoclonal antibody, mix and let stand for 10 minutes, add 10 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, and redissolve the precipitate with 1000 μL reconstitution solution;

[0055] ②Take 1ml of colloidal gold, add 2-10μL of 0.2M K 2 CO 3 After mixing, add 10-30 μg CRE monoclonal antibody, mix and let stand for 10 minutes, add 10 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, and redissolve the precipitate with 1000 μL reconstitution solution;

[0056] ③Take 2ml of colloidal gold, add 10μL of 0.2M K 2 CO 3 After mixing, add 30 μg rabbit antibody, mix and let stand for 10 minutes, add 20 μL 20% BSA to block, mix again and let stand for 10 minutes, centrifuge for 7 minutes (9000r), discard the supernatant, an...

Embodiment 3

[0062] The kit obtained above was tested, and a total of 25 healthy samples, 25 samples of rectal cancer patients, and 26 samples of gastric cancer patients were detected, and the sample volume was 80 μL. Product curve (see Figure 8) and product application test statistics (see Figure 9 and Table 1).

[0063] Table 1

[0064]

[0065] Carry out product curve by the detection result of above-mentioned Fig. 8, pass Y=A+C / [1+exp{-B(X-D)}] and Y=(A-D) / [1+(X / C)^B]+ D fitting gives R 2 ≥0.9801 by R 2 It can be seen that each index detected by the obtained kit of the present invention meets the requirements for use of the product.

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Abstract

The invention relates to a biological detection method, and particularly relates to a diacetyl spermine detection kit and a preparation method thereof. The diacetyl spermine detection kit comprises a test strip, wherein the test strip sequentially comprises a glass cellulose film, a gold pad, an NC film and absorbent paper from a sample charging end. The diacetyl spermine detection kit is characterized in that monoclonal antibodies of DiAcSpm and CRE labeled by colloidal gold and a rabbit antibody are coated on the gold pad; a detection region (T) on the NC film has antigens of the DiAcSpm and the CRE, and a standard region (C) has a goat anti rabbit antibody. According to the diacetyl spermine detection kit, by applying principles of cytobiology, molecular biology, biochemistry, immunology and the like, using a colloidal gold immunochromatography technology, and adopting single methods such as urinalysis and the like which are easy to operate and noninvasive, a diacetyl spermine in vitro diagnosis kit for early discovery and early diagnosis of diacetyl spermine in urine of patients suffering from multiple cancers can be obtained. The detection is performed by using a colloidal gold immunochromatography analyzer, and the purposes of rapidness, accuracy and convenience in operation are achieved.

Description

technical field [0001] The invention relates to a biological detection method, and is characterized in that it is specifically a detection kit for diacetylspermine, a preparation method and a method. Background technique [0002] Diagnostic reagents play a great role in the prevention and diagnosis of diseases. As a sub-industry of biopharmaceuticals, during the development of more than 20 years, the scope of application of diagnostic reagents has continued to expand, and they are playing an important role in the prevention and detection of diseases. The role is getting bigger and bigger, and the commercial value is also constantly highlighted. In 2010, my country's diagnostic reagent industry was under the background of the new medical reform and the state's key support for the development of the diagnostic reagent industry in the field of biomedicine. The market size of my country's diagnostic reagents reached about 4 billion yuan. At present, the market share of my count...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531G01N33/574
CPCG01N33/57488G01N33/577
Inventor 李文欣刘峰
Owner LIAONING MEDI BIOTECH CO LTD
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