Method for rapidly propagating dendrobium chrysotoxumLindl. seeds by tissue culture

A technology of Dendrobium Dendrobium and tissue culture is applied in the field of rapid propagation of Dendrobium Dendrobium seeds, which can solve problems such as labor and achieve the effect of improving the efficiency of tissue culture.

Active Publication Date: 2015-02-04
江苏北环生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of Dendrobium chrysalis seed tissue culture rapid propagation method, directly take the seed of Dendrobium chrysalis for tissue culture, effectively shorten the culture cycle of Dendrobium chrysalis, reduce the variation probability caused by multiple subcultures , the tissue-cultured seedlings of Dendrobium chrysalis obtained by tissue culture are strong and strong, and are suitable for industrial production to solve the seedling problem of artificial planting of Dendrobium chrysalis

Method used

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  • Method for rapidly propagating dendrobium chrysotoxumLindl. seeds by tissue culture

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Experimental program
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Effect test

Embodiment 1

[0027] Step 1. The method of obtaining the aseptic material of Dendrobium chrysalis: Before the seeds of Dendrobium chrysalis become yellow and not cracked, take the fruit, clean it under running water, soak it in 75% ethanol for 30 seconds, disinfect it with mercury chloride for 15 minutes, and wash it with sterile water 5 times, use a scalpel to cut the fruit in the sterilized tray, take out the seeds, and sow them in the induction medium. Indoleacetic acid 2.0mg / L, thiadizuron 0.5mg / L, 35g / L sucrose and 5g / L agar, and the pH value of the induction medium was adjusted to 5.8, the culture temperature was 28°C, the light intensity was 2000lux, and the light time was After culturing for 15 days under the condition of 13h / d, small buds of Dendrobium chrysalis can be seen to emerge, and small buds of Dendrobium chrysalis can be obtained, and the seed germination rate is 96%.

[0028] Step 2, placing a large amount of small buds of Dendrobium chrysalis in the subculture medium, th...

Embodiment 2

[0032] Step 1. Before the seeds of Dendrobium chrysanthemum are yellow and not cracked, take the fruit, clean it under running water, soak it in 80% ethanol for 40 seconds, disinfect it with mercury chloride for 20 minutes, wash it 6 times with sterile water, and use it in a sterilized tray. Cut the fruit with a scalpel, take out the seeds, and sow them in the induction medium. The culture temperature is 30°C, the light intensity is 2300lux, and the light time is 14h / d. After 15 days of cultivation, small buds can be seen from Dendrobium chrysalis, and you can get Drumstick Dendrobium small buds; the induction medium is: MS-H is used as the basic medium, and then 2.0mg / L naphthaleneacetic acid, 2.0mg / L indoleacetic acid, 1.0mg / L thiadizuron, 25-35g / L sucrose are added And 3-5g / L agar, and adjust the pH value of the induction medium to 5.9. The seed germination rate was 97%.

[0033] Step 2, placing the small buds of Dendrobium chrysanthemum in the subculture medium, the subcu...

Embodiment 3

[0037] Step 1. Before the seeds of Dendrobium chrysanthemum are yellow and not cracked, take the fruit, clean it under running water, soak it in 80% ethanol for 40 seconds, disinfect it with mercury chloride for 20 minutes, wash it 6 times with sterile water, and use it in a sterilized tray. Cut the fruit with a scalpel, take out the seeds, and sow them in the induction medium. The culture temperature is 30°C, the light intensity is 2500lux, and the light time is 14h / d. After 15 days of cultivation, small buds can be seen from Dendrobium chrysalis, and you can get Drumstick Dendrobium small buds; wherein the induction medium is: take MS-H as the basic medium, then add 1.5mg / L naphthaleneacetic acid, 1.0mg / L indoleacetic acid, 1.5mg / L thiadidiazuron, 35g / L sucrose and 3 -5g / L agar, and adjust the pH value of the induction medium to 5.5-6. The seed germination rate was 98%.

[0038] Step 2, placing the small buds of Dendrobium chrysotoxum in the subculture medium, the subcultur...

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Abstract

The invention discloses a method for propagating dendrobium chrysotoxumLindl. seeds by tissue culture, which comprises the following steps: seeding the dendrobium chrysotoxumLindl. seeds into an induction medium for induced germination; then successively transferring the dendrobium chrysotoxumLindl. budlets to a subculture medium and a rooting medium for subsequent cultivation so as to obtain the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings; and then transplanting the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings to greenhouses for seedling hardening. According to the tissue culture method disclosed by the invention, the steps are simple, the operation is convenient, the obtained dendrobium chrysotoxumLindl. rooting tissue cultured seedlings are strong; the growing ability is high, the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings are suitable for industrial production.

Description

technical field [0001] The invention relates to a method for rapid propagation of plants, in particular to a method for rapid propagation of Dendrobium chrysanthemum seed tissue culture. Background technique [0002] Dendrobium chrysotoxum Lind1. is a perennial epiphytic herb of Orchidaceae. Pseudobulbs are erect, fleshy, spindle-shaped or drumstick-shaped, with 2-5 nodes and most blunt ribs, golden yellow after drying; leaves are terminal, leathery, 2-5 pieces, oblong, 10-19cm long, 2-4cm wide, sharp and hooked at the apex. The raceme is extracted from the leaf axil near the top, up to 20cm long, with 5-8 sparse flowers, golden yellow, with a delicate fragrance; the lip is nearly kidney-shaped and round, with red stripes on both sides of the base, and a deep red halo in the center, The edge is wavy, densely covered with short hairs, and the flowering period is March-May. Dendrobium chrysalis is native to the south to southwest of Yunnan, my country, and grows at an altit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 霍丽妮苏钛陈睿李培源苏炜吕金燕
Owner 江苏北环生物科技有限公司
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