Method for extracting high-activity lumbrukinase from earthworm

A lumbrokinase and high activity technology, which is applied in the field of biochemical pharmacy, can solve the problems of low lumbrokinase production capacity, many waste liquids and high preparation cost of lumbrokinase, and achieves the effect of high effective components of extracts

Inactive Publication Date: 2015-02-11
TIANJIN JIALIMING EARTHWORM BREEDING
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of lumbrokinase in the prior extraction method is very low, and the preparation cost of lumbrokinase is

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] A method for extracting highly active lumbrokinase from earthworms, comprising the following steps:

[0024] (1) Select earthworms

[0025] Choose high-quality earthworm varieties, which cannot contain heavy metals in their bodies;

[0026] (2) cleaning

[0027] Take a certain amount of live earthworms, wash them several times with water, remove the dirt in their viscera, screen out and rinse various impurities and drain the sewage;

[0028] (3) Refining

[0029] A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for extracting high-activity lumbrukinase from earthworms comprises the following steps: (1) choosing earthworms; (2) cleaning; (3) refining; (4) centrifuging with high speed centrifugal machine, removing impurities in the slurry after salting, collecting supernatant, washing the precipitate twice with salt solution, centrifuging again, collecting supernatant again, and mergingthe collected supernatant; (5) conducting first ultrafiltration on the merged supernatant to filter out high molecules insoluble in water; conducting ultrafiltration on the supernatant subjected to ultrafiltration, concentrating the effective components, and when the concentrate reaches a concentration of 5%-10%, sending the concentrate into a vacuum freeze dryer; (6) conducting vacuum freeze drying; and (7) packaging. The invention can obtain all lumbrukinase from earthworms by one step, has the characteristics of simpleness, feasibility and high effective components in the extract, and is applicable to mass production.

Description

technical field [0001] The invention belongs to the technical field of biochemical pharmacy, and specifically relates to a method for extracting highly active lumbrokinase from earthworms. Background technique [0002] The high morbidity, high mortality and high disability of thrombotic diseases seriously threaten the survival of human beings, and thrombolytic drugs are the first choice for the treatment of this disease. At present, there are common side effects that easily cause bleeding in the thrombolytic drugs used clinically; some are expensive and unbearable for ordinary people; some have poor stability and require high storage conditions, which brings a lot of inconvenience to drug users; The kind of drug administration mode is single, only has oral, or only has intravenous or intramuscular injection, and the scope of use medicine is very limited. Lumbrokinase is an active substance extracted from earthworms. It has been proven to have good clinical application valu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/68
CPCC12N9/6408C12Y304/21007
Inventor 贾立明王凤艳贾京华刘平陈小明
Owner TIANJIN JIALIMING EARTHWORM BREEDING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap