Method for extracting high-activity lumbrukinase from earthworm

Abstract

A method for extracting high-activity lumbrukinase from earthworms comprises the following steps: (1) choosing earthworms; (2) cleaning; (3) refining; (4) centrifuging with high speed centrifugal machine, removing impurities in the slurry after salting, collecting supernatant, washing the precipitate twice with salt solution, centrifuging again, collecting supernatant again, and mergingthe collected supernatant; (5) conducting first ultrafiltration on the merged supernatant to filter out high molecules insoluble in water; conducting ultrafiltration on the supernatant subjected to ultrafiltration, concentrating the effective components, and when the concentrate reaches a concentration of 5%-10%, sending the concentrate into a vacuum freeze dryer; (6) conducting vacuum freeze drying; and (7) packaging. The invention can obtain all lumbrukinase from earthworms by one step, has the characteristics of simpleness, feasibility and high effective components in the extract, and is applicable to mass production.

Description

technical field [0001] The invention belongs to the technical field of biochemical pharmacy, and specifically relates to a method for extracting highly active lumbrokinase from earthworms. Background technique [0002] The high morbidity, high mortality and high disability of thrombotic diseases seriously threaten the survival of human beings, and thrombolytic drugs are the first choice for the treatment of this disease. At present, there are common side effects that easily cause bleeding in the thrombolytic drugs used clinically; some are expensive and unbearable for ordinary people; some have poor stability and require high storage conditions, which brings a lot of inconvenience to drug users; The kind of drug administration mode is single, only has oral, or only has intravenous or intramuscular injection, and the scope of use medicine is very limited. Lumbrokinase is an active substance extracted from earthworms. It has been proven to have good clinical application valu...

AI Technical Summary

Problems solved by technology

However, the yield of lumbrokinase in the prior extraction method is very low, and the preparation cost of lumbrokinase is very high, resulting in a lot of waste liquid to be treated, and the production capacity of lumbrokinase is relatively low.