Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and application of extracting glycoprotein by using sea cucumber industrial blanching liquid

A blanching liquid and glycoprotein technology, applied in the preparation method of peptides, peptide/protein components, chemical instruments and methods, etc., can solve the problem of glycoproteins increasing the number of white blood cells, so as to reduce the number of white blood cells and reduce the use of The effect of increasing the amount and improving the level of high-value utilization

Inactive Publication Date: 2017-10-27
BIOLOGY INST OF SHANDONG ACAD OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among the many sea cucumber active substances, there is no literature report that the glycoprotein extracted from sea cucumber blanching liquid can significantly increase the number of white blood cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and application of extracting glycoprotein by using sea cucumber industrial blanching liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting glycoproteins by using processed sea cucumber blanching liquid, which is characterized in that it comprises the following steps: (1) collecting 4000 L of processed sea cucumber blanching liquid, concentrating under reduced pressure until the volume is 300 L, and the solid content is about 35%.

[0037] (2) The concentrated solution is spray-dried to obtain a solid powder;

[0038] (3) Add the powder into 500L 80% ethanol solution, stir for desalination and saponin removal, after stirring for 24 hours, centrifuge with a three-legged centrifuge at a speed of 1850rpm, and collect the precipitate by centrifugation;

[0039] (4) Precipitation Repeat step (3) 3 times to obtain a precipitate that has been desalted and desaponinized 4 times;

[0040] (5) Add the above precipitate into 500L 90% ethanol solution, stir for 24 hours, remove fat, use suction filtration equipment to collect the filter residue, and dry the filter residue to obtain a solid powde...

Embodiment 2

[0045] A method for extracting glycoproteins by using processed sea cucumber blanching liquid, which is characterized in that it comprises the following steps: (1) collecting 4000 L of processed sea cucumber blanching liquid, concentrating under reduced pressure until the volume is 300 L, and the solid content is about 35%.

[0046] (2) The concentrated solution is spray-dried to obtain a solid powder;

[0047] (3) Add the powder into 500L 75% ethanol solution, stir for desalination and saponin removal, after stirring for 24 hours, use suction filtration equipment to collect the filter residue;

[0048] (4) Add the filter residue to 500L 75% ethanol solution, repeat step (3) 3 times, and obtain a filter residue that has been desalted and desaponinized 4 times;

[0049] (5) Add the above filter residue into 500L 95% ethanol solution, stir for 24 hours, remove the fat, use suction filtration equipment to collect the filter residue, and dry the filter residue to obtain a solid powd...

Embodiment 3

[0053] Embodiment 3: The similarities between this embodiment and Embodiment 1 will not be repeated. The difference lies in that the use of sea cucumber processed blanching liquid to extract glycoprotein is used as a drug for the preparation of white blood cells.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for extracting glycoprotein by utilizing a sea cucumber treatment blanching liquid and application thereof. The method for extracting the glycoprotein by utilizing the sea cucumber treatment blanching liquid comprises the following steps: (1) concentrating the sea cucumber treatment blanching liquid to obtain a concentrated liquid with the solid content of 15-45%; (2) drying the concentrated liquid to obtain a first solid powder; (3) adding the solid powder to 70-85% ethanol solution, stirring, desalting, removing saponin, and centrifuging or extraction filtering to collect a first solid insoluble substance; (4) repeatedly performing the steps (3) on the first solid insoluble substance for twice or three times to obtain a second solid insoluble substance without salt and the saponin; (5) adding the second solid insoluble substance to a 90-95% ethanol solution to stir, degreasing, extraction filtering to collect a third solid insoluble substance, and drying the third solid insoluble substance to obtain a second solid powder; (6) adding the second solid powder to purified water, heating and extracting to obtain an extract solution; (7) centrifuging the extract solution, removing sediment, filtering liquid supernatant, collecting filtrate, and drying the filtrate to obtain the glycoprotein with function of increasing white blood cells. The method for extracting the glycoprotein by utilizing the sea cucumber treatment blanching liquid is applied to preparation of medicines for increasing the white blood cells.

Description

technical field [0001] The invention relates to a method for extracting glycoproteins that can significantly increase leukocytes by using sea cucumber processed blanching liquid. Background technique [0002] After fresh sea cucumbers are harvested, due to the presence of proteolytic enzymes such as autolytic enzymes, it is easy to peel and autolyze. In order to facilitate preservation, fresh sea cucumbers are usually processed and stored after blanching in hot water. However, fresh sea cucumber produces a large amount of blanching liquid in the process of hot water treatment, which contains a large amount of active substances such as sea cucumber saponins (Separation and Identification of Several Sea Cucumber Saponin Compounds in Apostichopus japonicus Processing Waste Liquid)Journal of Shandong University ( Science Edition) Issue 7, 2011), sea cucumber glycoprotein (ZL 20111 0384722.8), etc. [0003] Sea cucumber saponins have strong hemolytic, cytotoxic, in vitro anti-t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/14A61K38/17A61P7/00
CPCA61K38/00C07K14/43504
Inventor 袁文鹏刘昌衡张绵松张永刚夏雪奎贾爱荣胡炜刘新史亚萍乔若瑾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com