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Preparation method of laver oligosaccharide and application of laver oligosaccharide in fish feed

A technology of laver oligosaccharides and fish feed, which is applied in application, animal feed, animal husbandry, etc., can solve the problems of browning, incomplete reaction, and rising cost of products, so as to enhance immunity, increase added value, and save cost effect

Active Publication Date: 2021-06-29
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrogen peroxide degradation has the best prospect in the chemical degradation method. This method is low in cost and easy to realize industrial production. However, the oxidizing agent does not fully participate in the reaction process. At the same time, the product is more prone to browning as the temperature rises; The reaction can effectively avoid side effects, and the external reaction conditions are also easy to control, but the use of enzyme reactions leads to increased costs; the degradation reaction by physical methods is not complete

Method used

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  • Preparation method of laver oligosaccharide and application of laver oligosaccharide in fish feed
  • Preparation method of laver oligosaccharide and application of laver oligosaccharide in fish feed
  • Preparation method of laver oligosaccharide and application of laver oligosaccharide in fish feed

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of laver oligosaccharide

[0034] Method 1. Biochemical synergistic biocatalytic high-efficiency preparation technology of laver oligosaccharides (the method used in the patent of the present invention)

[0035] ① Preparation of immobilized enzyme catalyst (enzyme-MCM-48)

[0036] Weigh 3 g of cellulase and add it to 50 mL of acetic acid-sodium acetate buffer solution (pH=4.5), stir for 1 h, centrifuge to collect the supernatant, continue stirring for 1 h and centrifuge the precipitate until it is completely dissolved, and dilute the solution to 100 mL to obtain Cellulase enzyme solution. Weigh 10 g of dry MCM-48 molecular sieves and immerse in 100 mL of 3% cellulase solution, stir at room temperature for 12 h, centrifuge and wash with distilled water. The precipitate was vacuum-dried to obtain the immobilized enzyme catalyst.

[0037] ② Preparation of molecular sieve-supported Fenton catalyst (Fe-MCM-48)

[0038]Prepare 100 mL of 2M fe...

Embodiment 2

[0047] Embodiment 2, the preparation of laver oligosaccharide feed (PS, PO), laver polysaccharide feed (PP) and laver powder feed (PM)

[0048] The laver oligosaccharide extract (OP) that method one of embodiment 1 obtains is added in the feed by 0.05-5% of feed gross weight, any concentration in this range can significantly improve the meat quality and local flavor of farmed fish, promote Cellular immunity, improve its ability to resist diseases, only several different concentrations are used as representative samples in the examples. The preparation method is as follows: first make a solution of 8-12% of laver oligosaccharide extract, preferably 10%; then add absolute ethanol under stirring so that the total concentration of ethanol is 10%-20%, preferably 15%; The obtained solution is evenly sprayed on the feed to prepare a feed in which laver oligosaccharides account for 0.1% of the total weight of the feed, which is dried or dried and stored for later use, which is recorde...

Embodiment 3

[0049] Example 3, the effect of feeding aquatic feed containing laver oligosaccharides on grouper meat quality improvement and immune regulation

[0050] In this experiment, the grouper was fed with aquatic feed containing laver oligosaccharides, and its effects on the improvement of grouper meat quality and immune regulation were explained through the changes in grouper body composition, muscle texture, and physiological function-related indicators.

[0051] Test method: Select healthy and uniform grouper and divide them into 3 groups at random, use different baits respectively, and carry out according to normal feeding and management procedures. The first group is the commercially available aquatic feed F control group, the second group is the PM control group, and the third group is the PS experimental group. The test period is 42 days. The blood is collected from 9 fish every 14 days, and the head kidney is taken aseptically. Used to measure the respiratory burst of head k...

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Abstract

The invention provides a preparation method of laver oligosaccharide and application of the laver oligosaccharide in fish feed. The laver oligosaccharide is prepared from through a biochemical synergistic efficient catalysis method, the method is simple to operate and high in yield, and sulfate groups in sugar chains are reserved to the maximum extent. The laver oligosaccharide is mainly formed by connecting 3-connected beta-D-galactose and 4-connected alpha-L-galactose-6-sulfo group, the degree of polymerization is 2 to 20, the content of galactose is 60 to 80 percent, and the content of sulfo group is 15 to 30 percent. The laver oligosaccharide is added into the fish feed, so that the meat quality of cultured fishes can be improved, the fat content of fish bodies is reduced, the protein content of the fish bodies is increased, and the meat quality and flavor of the fish bodies are improved. The laver oligosaccharide is a brand-new, green and safe fish feed additive, can effectively solve the problems of loose meat quality, low protein content and poor taste of cultured fishes, can improve the immunity of the fishes, and can broaden the market for the cultured fishes.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a preparation method of laver oligosaccharide and its application in fish feed. Background technique [0002] With the development of the aquaculture industry, the problems restricting the production of aquaculture have become increasingly prominent. One of the current problems that seriously affects the sales of farmed fish is that the flavor of farmed fish is poor. Severe fishy smell, loose meat quality, and high fat content all make consumers stay away. The lower quality of farmed fish has seriously affected its commercial value and development. The poor meat quality of farmed fish is directly related to the harsh farming environment and low-quality feed. The polluted water environment will inevitably lead to the accumulation of fish diseases and fishy smell substances, while the ordinary formula feed has high energy, resulting in high body fat and low lean meat percentage of fish...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23K20/163A23K50/80
CPCC08B37/0003C08B37/006A23K20/163A23K50/80
Inventor 王晶王晓晴张全斌刘婧吴宁耿丽华岳洋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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