Preparation method of laver oligosaccharide and application of laver oligosaccharide in fish feed
A technology of laver oligosaccharides and fish feed, which is applied in application, animal feed, animal husbandry, etc., can solve the problems of browning, incomplete reaction, and rising cost of products, so as to enhance immunity, increase added value, and save cost effect
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Embodiment 1
[0033] Embodiment 1, the preparation of laver oligosaccharide
[0034] Method 1. Biochemical synergistic biocatalytic high-efficiency preparation technology of laver oligosaccharides (the method used in the patent of the present invention)
[0035] ① Preparation of immobilized enzyme catalyst (enzyme-MCM-48)
[0036] Weigh 3 g of cellulase and add it to 50 mL of acetic acid-sodium acetate buffer solution (pH=4.5), stir for 1 h, centrifuge to collect the supernatant, continue stirring for 1 h and centrifuge the precipitate until it is completely dissolved, and dilute the solution to 100 mL to obtain Cellulase enzyme solution. Weigh 10 g of dry MCM-48 molecular sieves and immerse in 100 mL of 3% cellulase solution, stir at room temperature for 12 h, centrifuge and wash with distilled water. The precipitate was vacuum-dried to obtain the immobilized enzyme catalyst.
[0037] ② Preparation of molecular sieve-supported Fenton catalyst (Fe-MCM-48)
[0038]Prepare 100 mL of 2M fe...
Embodiment 2
[0047] Embodiment 2, the preparation of laver oligosaccharide feed (PS, PO), laver polysaccharide feed (PP) and laver powder feed (PM)
[0048] The laver oligosaccharide extract (OP) that method one of embodiment 1 obtains is added in the feed by 0.05-5% of feed gross weight, any concentration in this range can significantly improve the meat quality and local flavor of farmed fish, promote Cellular immunity, improve its ability to resist diseases, only several different concentrations are used as representative samples in the examples. The preparation method is as follows: first make a solution of 8-12% of laver oligosaccharide extract, preferably 10%; then add absolute ethanol under stirring so that the total concentration of ethanol is 10%-20%, preferably 15%; The obtained solution is evenly sprayed on the feed to prepare a feed in which laver oligosaccharides account for 0.1% of the total weight of the feed, which is dried or dried and stored for later use, which is recorde...
Embodiment 3
[0049] Example 3, the effect of feeding aquatic feed containing laver oligosaccharides on grouper meat quality improvement and immune regulation
[0050] In this experiment, the grouper was fed with aquatic feed containing laver oligosaccharides, and its effects on the improvement of grouper meat quality and immune regulation were explained through the changes in grouper body composition, muscle texture, and physiological function-related indicators.
[0051] Test method: Select healthy and uniform grouper and divide them into 3 groups at random, use different baits respectively, and carry out according to normal feeding and management procedures. The first group is the commercially available aquatic feed F control group, the second group is the PM control group, and the third group is the PS experimental group. The test period is 42 days. The blood is collected from 9 fish every 14 days, and the head kidney is taken aseptically. Used to measure the respiratory burst of head k...
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