A standardized, high-precision, and general-purpose method for building functional modules

A functional module and high-precision technology, applied in the field of synthetic biology, can solve problems that do not involve the assembly of multiple modules
CN104419716BActive Publication Date: 2017-12-29TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2017-12-29

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Abstract

The invention discloses a standardized, high-precision, and general-purpose functional module construction method, which includes the following steps: (1) Adding standardized sites to the source gene to form a genetic element, and connecting the genetic element to the plasmid pLD-Blunt to form a genetic (2) Get the gene element from the plasmid with the gene element and connect it to the following gap in the module tool plasmid: terminator 1-promoter-AATG-gap-TAAA-terminator 2 ; Construct plasmids with different functional modules; (3) Transfer different functional modules from plasmids with different functional modules, and co-transform S. The functional modules are stored in the plasmid; the present invention has versatility, standardization, high precision and free selectivity.
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Description

technical field

[0001] The invention relates to the field of synthetic biology, in particular to a method for constructing functional modules. Background technique

[0002] Functional modules play a crucial role in synthetic biology research. Artificial optimization and reconstruction of endogenous metabolic pathways, transformation of chassis cell genomes, rapid assembly of multiple exogenous genetic elements, and artificial design and construction of new life functions all rely on functional modules. Therefore, designing a set of standardized, high-precision, and general-purpose functional module construction and assembly methods, so that it can quickly and efficiently obtain the required modules, has become an urgent bottleneck technology for synthetic biology.

[0003] The ideal construction method of functional modules urgently needed by synthetic biology should have the following salient features: 1) For any genetic element, no site pre-modification is required. 2) D...

Claims

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