Sterilization composition containing picoxystrobin and difenoconazole and application of composition
The technology of a difenoconazole and fungicide composition is applied in the field of pesticides, which can solve the problems of easily causing phytotoxicity, large environmental pollution, and easy residues in crops, etc., and achieves sustainable development, reduced harm, and good The effect of economic benefits
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[0018] Bioassay Example 1: Determination of the virulence of picoxystrobin and difenoconazole against Apple anthracnose
[0019] The indoor virulence of a mixture of picoxystrobin and difenoconazole on apple anthracnose bacteria was studied by the mycelial growth rate method. The experimental method used Sun Yunpei’s method to calculate the co-toxicity coefficient (CTC) to evaluate the mixed effect. When the CTC value is greater than 120, the mixture is considered to have synergistic effect, when it is less than 80, it is considered to be antagonistic, and when it is between 80 and 120, it is considered to have an additive effect. The specific method is as follows:
[0020] Take the prepared drug solution and place it in 100ml of 50-60℃ PDA sterilized medium, shake it, and pour it into a sterile petri dish with a diameter of 60mm. After cooling and solidification, transfer the bacteria cakes with a uniform growth and a diameter of 4mm. The sterile medium added with the same amount...
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