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A kind of preparation method of high-content Armillaria chrysanthemum polysaccharide

A yellow-green Armillaria, high-content technology is applied in the field of preparation of high-content polysaccharides, can solve the problems of high energy consumption, unspecified polysaccharide content and polysaccharide extraction rate, backwardness, etc., and achieves simple preparation process, no toxic side effects, less pollution

Active Publication Date: 2016-09-14
QINGHAI GAODEWEI BIOLOGICAL HEALTH PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the extraction methods of Armillaria chrysanthemum polysaccharides are boiling extraction, which is relatively backward and consumes a lot of energy
The patent "An anti-cancer Armillaria chrysanthemum polysaccharide and its extraction process" uses ultrasonic extraction to extract polysaccharides from Armillaria chrysogenum. The extracted polysaccharide does not indicate the polysaccharide content and polysaccharide extraction rate, but only points out that the polysaccharide has anti-cancer properties active

Method used

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  • A kind of preparation method of high-content Armillaria chrysanthemum polysaccharide

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A method for preparing a high-content Armillaria chrysanthemum polysaccharide, comprising the following steps:

[0034] (1) After drying the fresh Armillaria chrysanthemum at 30° C. to a constant weight, the dried Armillaria chrysanthemum is obtained.

[0035] (2) The dried Armillaria chrysanthemum was pulverized into a dry powder with a particle size of 10 um with a superfine pulverizer.

[0036] (3) Add water to the dry powder at a liquid-to-solid ratio of 10ml:1g, and then use microwave extraction method to extract for 15min under the conditions of microwave power of 50W and extraction temperature of 40°C to obtain the extract.

[0037] (4) The extract is concentrated under reduced pressure at a vacuum degree of 0.01 MPa and a temperature of 50° C., and then dehydrated to an extract to obtain a concentrated solution.

[0038] (5) Add 3 times the volume of absolute ethanol to the concentrated solution for precipitation, centrifuge after standing still for 2...

Embodiment 2

[0040] Example 2 A method for preparing high-content Armillaria chrysanthemum polysaccharides, comprising the following steps:

[0041] (1) After drying the fresh Armillaria chrysanthemum at 70° C. to a constant weight, the dried Armillaria chrysanthemum is obtained.

[0042] (2) The dried Armillaria chrysanthemum was pulverized into a dry powder with a particle size of 200 um with a superfine pulverizer.

[0043] (3) Add water to the dry powder at a liquid-to-material ratio of 100ml:1g, and then use microwave extraction method to extract for 60 minutes under the conditions of microwave power 1000W and extraction temperature 90°C to obtain the extract.

[0044] (4) The extract is concentrated and dehydrated under reduced pressure under the conditions of a vacuum of 0.09MPa and a temperature of 90°C until it is in the form of an extract to obtain a concentrated solution.

[0045] (5) Add 6 times the volume of absolute ethanol to the concentrated solution for precipitation, cen...

Embodiment 3

[0047] Example 3 A method for preparing a high-content Armillaria chrysanthemum polysaccharide, comprising the following steps:

[0048] (1) After drying the fresh Armillaria chrysanthemum at 40° C. to a constant weight, the dried Armillaria chrysanthemum is obtained.

[0049] (2) The dried Armillaria chrysanthemum was pulverized into a dry powder with a particle size of 100 um with a superfine pulverizer.

[0050] (3) Add water to the dry powder at a liquid-to-solid ratio of 50ml:1g, and then use microwave extraction method to extract for 30min under the conditions of microwave power of 500W and extraction temperature of 60°C to obtain the extract.

[0051] (4) The extract is concentrated by a nanofiltration membrane and then dehydrated into an extract to obtain a concentrate.

[0052] Among them: the membrane separation operating pressure is 20 MPa.

[0053] (5) Add 4 times the volume of absolute ethanol to the concentrated solution for precipitation, centrifuge after stan...

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Abstract

The invention relates to a preparation method of high-content armillaria luteo-virens polysaccharide. The preparation method comprises the following steps: (1) drying fresh armillaria luteo-virens to constant weight to obtain dried armillaria luteo-virens; (2) crushing the dried armillaria luteo-virens to obtain dried powder; (3) adding the dried powder into water and then extracting by virtue of a microwave extraction method to obtain an extraction liquid; (4) concentrating the extraction liquid at a reduced pressure or concentrating the extraction liquid by an organic film, dehydrating the concentrated extraction liquid into an extract to obtain a concentrated solution; (5) precipitating the concentration solution with anhydrous ethanol, standing for 24 hours, then centrifugalizing, and collecting a precipitate A to obtain armillaria luteo-virens polysaccharide; and (6) dissolving the armillaria luteo-virens polysaccharide by pure water, filtering to obtain supernate, precipitating the supernate with anhydrous ethanol, standing for 24 hours, then centrifugalizing, collecting the precipitate B, and drying the precipitate B to constant weight to obtain the armillaria luteo-virens polysaccharide with the polysaccharide content which is greater than 80%. The preparation method provided by the invention not only is short in extraction time and high in extraction rate, but also is relatively high in polysaccharide content and energy-saving and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of high-content polysaccharide, in particular to a preparation method of high-content Armillaria chrysanthemum polysaccharide. Background technique [0002] Armillaria luteo-vriens (Amillarialuteo-vriens), also known as chanterelle, belongs to Basidiomycotina, Phytomycetes, Trichomonas, Tricholomaceae, Armillaria. Armillaria chrysanthemum is rich in polysaccharides, proteins, amino acids, etc. It has the effects of enhancing the body's immune function and inhibiting tumor growth, and has high nutritional value and therapeutic health effects. [0003] Microwave extraction is a new type of extraction technology developed in recent years, which has the advantages of short extraction time, high extraction efficiency, energy saving and environmental protection. At present, most of the extraction methods of Armillaria chrysanthemum polysaccharides are boiling extraction, which is relatively backward and consumes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 陈晨邵赟陶燕铎文怀秀
Owner QINGHAI GAODEWEI BIOLOGICAL HEALTH PROD CO LTD
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