Pneumonia mycoplasma medium

A technology of Mycoplasma pneumoniae and liquid culture medium, which is applied in the field of microbial detection, can solve the problems of time-consuming and false-positive liquid culture methods, and achieve the effects of speeding up diagnosis, improving specificity, and facilitating popularization and application

Active Publication Date: 2015-03-25
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a new Mycoplasma pneumoniae culture medium, which is a new Mycoplasma pneumoniae culture medium, aimed at the current detection of Mycoplasma pneumoniae, the liquid culture method has false positives, and the detection of solid medium is too time-consuming. Mycoplasma pneumoniae biphasic medium

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Experimental program
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Effect test

Embodiment 1

[0040] The preparation of embodiment 1 Mycoplasma pneumoniae biphasic culture medium

[0041] The DMEM powder is dissolved in water to form a concentration of 10g / L for subsequent use. Weigh or measure 50g beef extract, 10g peptone, 5g sodium chloride, 100mL bovine serum, 10mL DMEM liquid culture medium, 10mL25% yeast extract, 5g glucose, 0.5mL0.4% phenol red indicator, 0.01g Nystatin, 1000IU penicillin, and 0.5g / L nicotinamide adenine dinucleotide were briefly mixed and added to 1000mL of distilled water to adjust the pH to 7.8, then sterilized by filtration with a 0.22μm bacterial filter membrane, and then Heat to 60°C to fully dissolve each component, and then divide the medium into two parts accounting for 3 / 4 and 1 / 4 of the total amount respectively, and add 11g agar powder to the part accounting for 3 / 4, after heating and dissolving, Transfer to a sterile transparent plastic tube with a diameter of 5 cm and a length of 10 cm, and tilt the plastic tube to cool to form a ...

Embodiment 2

[0042] The preparation of embodiment 2 Mycoplasma pneumoniae biphasic culture medium

[0043] The DMEM powder is dissolved in water to form a concentration of 10g / L for subsequent use. Weigh or measure 45g beef extract, 8g peptone, 5g sodium chloride, 80mL bovine serum, 12mL DMEM liquid culture medium, 12mL20% yeast extract, 4g glucose, 0.4mL0.3% phenol red indicator, 0.008g Nystatin, 800IU penicillin, and 0.5g / L nicotinamide adenine dinucleotide were briefly mixed and added to 1000mL of distilled water to adjust the pH to 7.5, then sterilized by filtration with a 0.22μm bacterial filter membrane, and then Heat to 60°C to fully dissolve each component, and then divide the medium into two parts accounting for 3 / 4 and 1 / 4 of the total amount respectively, and add 15g agar powder to the part accounting for 3 / 4, after heating and dissolving, Transfer to a sterile transparent glass tube with a diameter of 3 cm and a length of 7 cm, and tilt the glass tube to cool to form a slope, ...

Embodiment 3

[0044] The preparation of embodiment 3 Mycoplasma pneumoniae biphasic culture medium

[0045] The DMEM powder is dissolved in water to form a concentration of 10g / L for subsequent use. Weigh or measure 55g beef extract, 10g peptone, 6g sodium chloride, 110mL bovine serum, 18mL DMEM liquid culture medium, 10mL30% yeast extract, 6g glucose, 0.6mL0.6% phenol red indicator, 0.012g Nystatin, 1200IU penicillin and 1.0g / L nicotinamide adenine dinucleotide were briefly mixed and added to 1000mL distilled water to adjust the pH to 8.0, and then sterilized by filtration with a 0.22μm bacterial filter membrane, and then Heat to 60°C to fully dissolve each component, then divide the medium into two parts accounting for 3 / 4 and 1 / 4 of the total amount respectively, and add 18g agar powder to the part accounting for 3 / 4, after heating and dissolving, Transfer to a sterile transparent plastic tube with a diameter of 7 cm and a length of 15 cm, and tilt the plastic tube to cool to form a slo...

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Abstract

The invention discloses a pneumonia mycoplasma medium. According to the pneumonia mycoplasma medium, a container holds a solid medium and a liquid medium; the solid medium shows an inclined plane in the container, and 1/4-1/2 of the inclined plane is immersed in the liquid medium; the container also comprises a sealing member capable of sealing the internal space of the container; and the solid medium comprises compositions same to those of the liquid medium, besides 15-25 g/L of agar. The medium is used to detect pneumonia mycoplasma, helps to improve the detection specificity, is capable of obtaining a monoclonal bacterium colony and is convenient for downstream further research work based on the detection result.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a culture medium for mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae) belongs to ureaplasma, no cell wall and precursors, very few organelles. Mycoplasma pneumoniae mainly causes mycoplasma pneumonia. Although patients with mycoplasma pneumonia have severe symptoms, they generally have no obvious abnormal signs in chest physical examination. In addition to the manifestations of the respiratory system, mycoplasma pneumonia can be accompanied by multi-system and multi-organ damage. Since the clinical manifestations and chest X-ray examination of mycoplasma pneumonia are not characteristic, the diagnosis cannot be made based on clinical manifestations and chest X-ray examination alone. Definitive diagnosis requires detection of the pathogen. [0003] At present, the detection of mycoplasma pneumonia in China mainly relies on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/35
Inventor 刘锦铭朱长太郭健
Owner SHANGHAI PULMONARY HOSPITAL
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