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Carbon nano-tube procalcitonin detection kit and preparation method thereof

A technology for detecting kits and carbon nanotubes, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficulty in improving sensitivity, low sensitivity, and difficulty in detecting sensitivity

Inactive Publication Date: 2015-03-25
BENXI TAISITEJIE BIOTECH
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Problems solved by technology

Under the current technical conditions, it is difficult to improve the detection sensitivity by using tracer materials such as colloidal gold and nitrocellulose membrane as carriers.
[0003] There are many reasons for the low sensitivity, including raw materials, processes, etc., but the fundamental reason is the limitation of the colloidal gold method itself. It is difficult to improve the sensitivity by the traditional colloidal gold method.

Method used

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  • Carbon nano-tube procalcitonin detection kit and preparation method thereof

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Embodiment

[0033] 3. Preparation of carbon nanotube colloidal gold complex: take colloidal gold-labeled procalcitonin antibody, add 0.1 ml of the above-mentioned carbon nanotube dispersion, and stir for 30 minutes. Centrifuge at low temperature for 30min.

[0034] 4. Preparation of the carbon nanotube colloidal gold composite pad: Discard the supernatant, then add the gold standard diluent into the centrifuge tube, mix well, spray the above solution on the gold standard pad, and dry it for later use.

[0035] 5. Scribing film for test line and quality control line: Paste the NC film on the bottom plate for use. Prepare procalcitonin monoclonal antibody 2 at 0.5 mg / ml; prepare goat anti-mouse antibody at a concentration of 1.0 mg / ml; draw a film on the previously prepared NC membrane with a film spotter at 1.0 μl / cm, respectively. T, C line, dry at room temperature for 2 hours.

[0036] 6. Assembly: Assemble the dried colloidal gold pad, absorbent paper, sample pad, and bottom plate wit...

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Abstract

The invention discloses a carbon nano-tube procalcitonin detection kit and a preparation method thereof. A test paper base plate is sequentially adhered with a sample pad, a carbon nano-tube colloidal gold pad, a nitrocellulose membrane and a water absorbent pad. The carbon nano-tube procalcitonin detection kit is characterized in that the carbon nano-tube colloidal gold pad is adhered with a carbon nano-tube colloidal gold compound labeled procalcitonin monoclonal antibody 1, and the nitrocellulose membrane is coated with a detection line and a quality control line of a procalcitonin monoclonal antibody 2. According to the kit disclosed by the invention, procalcitonin in a sample is detected by adopting a carbon nano-tube colloidal gold labelling technology so as to judge whether bacterial infection occurs. The carbon nano-tube procalcitonin detection kit disclosed by the invention has the characteristics of simplicity in operation, rapid reaction, high sensitivity, strong specificity and the like, and is suitable for field detection and self-detection.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit, in particular to a carbon nanotube procalcitonin detection kit and a preparation method thereof, which are suitable for procalcitonin in clinical test samples. Background technique [0002] Colloidal gold immunochromatography technology is widely used in many fields such as disease marker detection, food detection, scientific research, etc. The tracer materials used are generally colloidal gold, latex particles, etc., and the chromatography material is generally cellulose membrane, including Nitrocellulose membrane, cellulose acetate membrane, mixed cellulose membrane, etc., among which nitrocellulose membrane is the most commonly used. Under the current technical conditions, it is difficult to improve the detection sensitivity by using tracer materials such as colloidal gold and nitrocellulose membrane as carriers. [0003] There are many reasons for the low sensitivity, including raw materials, pr...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531
CPCG01N33/6803G01N33/54346G01N33/558
Inventor 李洪江王岩董楷一李双才凤解雨婷徐志伟姜竹泉
Owner BENXI TAISITEJIE BIOTECH
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