Microscopic Imaging Optics for Imaging Flow Cytometry

Abstract

The invention discloses a microimaging optical system of an imaging flow cytometry and relates to the field of optical instruments applied to biology and medicine. The system solves the problems of an existing optical system of the imaging flow cytometry that the number of spectral imaging channels is small, imaging quality can not reach diffraction limit, and the resolution of the cytometry can not be improved. The system comprises a microobjective, a secondary imaging lens set, a multi-spectral light splitter, a multi-spectral imaging lens and a TDI camera. Full-wave band high-resolution amplification imaging of a sample to be tested is achieved through the achromatic microobjective, then an intermediate image plane is obtained through the secondary imaging lens set, a field stop is placed at the position of the intermediate image plane, collision is conducted through the secondary imaging lens set, stack splitting is conducted through the multi-spectral light splitter, and then light is converged on the TDI camera through the multi-spectral imaging lens. According to the system, full-spectrum subband multiple structures are adopted, it is guaranteed that the imaging quality of the imaging optical system can reach diffraction limit in any spectrum channel, and the minimum detection size of the whole system reaches 40 nm.

Description

Technical field [0001] The invention relates to the technical field of biological and medical optical instruments, in particular to a microscopic imaging optical system of an imaging flow cytometer. Background technique [0002] Flow cytometry is a technique for rapid quantitative analysis and sorting of cells or other biological particles (such as microspheres, bacteria, small model organisms, etc.) arranged in a single row in a liquid stream. In the fields of biology and medicine, when a large number of cells need to be scanned, the flow cytometer completely sacrifices spatial resolution and can obtain a high detection speed, tens of thousands of cells per second. The imaging flow cytometer can not only obtain the population analysis data of a large number of cells, but also can see the cell images in real time, and the analysis results of each step can be confirmed by the images. When it is necessary to obtain cell morphology and internal structure information, imaging flow c...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that the optical system of the existing imaging flow cytometer has fewer spectral imaging channels, the imaging quality cannot reach the diffraction limit, and the resolution of the cytometer cannot be improved, the present invention provides an imaging flow cytometer Microscopic Imaging Optical System

Images