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Method for rejuvenating dendrobium officinale tissue culture thin and weak seedlings

A culture medium and basal medium technology, applied in the field of plant tissue culture, can solve problems such as cost waste, achieve the effects of reducing production costs, improving effective utilization, and reducing production waste

Inactive Publication Date: 2015-04-01
云南红河州巨丰生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention proposes a rejuvenation method and formula for thin and weak seedlings of Dendrobium officinale, which avoids the waste of cost caused by discarding too many thin and weak seedlings in the process of transferring and cultivating seedlings

Method used

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  • Method for rejuvenating dendrobium officinale tissue culture thin and weak seedlings

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Embodiment Construction

[0007] Example of media formulation screening

[0008] The basic medium is MS medium, and the additives are potatoes and bananas. Using the four-factor five-level orthogonal design method, the amount of potatoes added is 0g / L, 20g / L, 40g / L, 60g / L, 80g / L; The added amount of NAA is 0g / L, 20g / L, 40g / L, 60g / L, 80g / L, and the added amount of NAA is 0 mg / L, 0.4 mg / L, 0.8 mg / L, 1.0 mg / L, 2.0 mg / L; the amount of 6-BA added is 0 mg / L, 1 mg / L, 1.5 mg / L, 2 mg / L, 4 mg / L; pH is 5.8, artificial auxiliary light 1200LUX, natural light 2000LUX, light and dark alternately The time is 12h / d. The temperature is 18-23°C. Consult the orthogonal table design method to design 25 groups of experiments in total, among which MS+NAA0.4mg / L+potato 80g / L+banana 40g / L+agar powder 6g / L+sucrose 30g / L strong seedling formula, its diameter can be increased by 40%, reaching Seedling diameter requirement.

[0009] Table 1 Orthogonal design table of four factors and five levels

[0010]

[0011] The resu...

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Abstract

The invention discloses a method for rejuvenating dendrobium officinale tissue culture thin and weak seedlings. The method is suitable for enabling dendrobium officinale tissue culture seedlings to be strong and rejuvenating thin and weak seedlings after planting culture, belonging to the field of plant biotechnology. According to the invention, strong seedlings and thin and weak seedlings which do not meet the requirement in planting culture for tissue culture seedlings are taken as objects; the transfer or germination standard can be reached by implanting a culture medium provided by the invention, so that the phenomenon that a great amount of thin and weak seedlings are discarded due to nonuniform growth and nutrient element absorption differences in the seedling tissue culture process is reduced effectively, and the production cost is reduced. By the tissue culture rapid propagation method provided by the invention and the rejuvenation culture for 65 days, the diameter can be thickened by about 40%, so that the effective seedling rate in the tissue culture seedlings is improved greatly, and the loss cost for the production of dendrobium officinale tissue culture seedlings is reduced.

Description

technical field [0001] The invention relates to a plant tissue culture method in plant biotechnology, in particular to a method for rejuvenating strong dendrobium officinale seedlings or weak seedlings after planting. Background technique [0002] Dendrobium officinale is the first of the nine great fairy grasses in China. It has extremely rich medicinal value, and there are very few wild resources. Due to long-term wild digging, wild Dendrobium officinale has reached an endangered state. The fruit setting rate of Dendrobium officinale is very low, the seeds are not easy to germinate, and the natural reproduction rate is extremely low. Establishing a tissue culture rapid propagation system can fundamentally solve this problem. [0003] So far, there have been many patents related to the tissue culture technology of Dendrobium candidum, most of which focus on the breeding formula and planting of Dendrobium candidum, but there is no professional research and patent on the med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 田晓玲杨晓寅马知遥杨瑞
Owner 云南红河州巨丰生物科技股份有限公司
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