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Method for extracting papaya seed meal polyphenol

A technology of papaya seeds and extractants, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., can solve the problems of resource waste and underutilization, and achieve low cost, high extraction rate and purity, The effect of short extraction time

Inactive Publication Date: 2015-04-01
宋鸟生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, papaya seed meal is rich in polyphenol active substances, but it has not been fully utilized, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for extracting polyphenols from papaya seed dregs, comprising the following steps:

[0018] (1) The papaya seed meal is washed to remove impurities, dried and placed in a pulverizer to pulverize, passed through an 80-mesh sieve and mixed evenly for later use;

[0019] (2) Accurately weigh the papaya seed meal powder, add acetone extractant with a concentration of 60% according to the ratio of solid to liquid at 1:8, and place it in an ultrasonic cleaner to extract 3 times, each extraction time is 20 minutes;

[0020] (3) After the extraction is completed, the extract is placed in a rotary evaporator at 50°C, concentrated under reduced pressure to remove acetone, and then eluted with double distilled water to remove impurities. After removing impurities, desorb with ethanol until the eluent is colorless. The eluate was concentrated and dried under reduced pressure to obtain polyphenols from papaya seed meal.

Embodiment 2

[0022] A method for extracting polyphenols from papaya seed dregs, comprising the following steps:

[0023] (1) The papaya seed meal is washed to remove impurities, dried and placed in a pulverizer to pulverize, passed through a 90-mesh sieve and mixed evenly for later use;

[0024] (2) Accurately weigh the papaya seed meal powder, add acetone extractant with a concentration of 80% according to the ratio of solid to liquid at 1:10, place it in an ultrasonic cleaner and extract it 4 times, and the extraction time is 40 minutes each time;

[0025] (3) After the extraction is completed, the extract is placed in a rotary evaporator at 50°C, concentrated under reduced pressure to remove acetone, and then eluted with double distilled water to remove impurities. After removing impurities, desorb with ethanol until the eluent is colorless. The eluate was concentrated and dried under reduced pressure to obtain polyphenols from papaya seed meal.

Embodiment 3

[0027] A method for extracting polyphenols from papaya seed dregs, comprising the following steps:

[0028] (1) The papaya seed meal is washed to remove impurities, dried and placed in a pulverizer to pulverize, passed through a 100-mesh sieve and mixed evenly for later use;

[0029] (2) Accurately weigh the papaya seed meal powder, add acetone extractant with a concentration of 70% according to the ratio of material to liquid at 1:12, and place it in an ultrasonic cleaner for extraction 5 times, each extraction time is 30 minutes;

[0030] (3) After the extraction is completed, the extract is placed in a rotary evaporator at 50°C, concentrated under reduced pressure to remove acetone, and then eluted with double distilled water to remove impurities. After removing impurities, desorb with ethanol until the eluent is colorless. The eluate was concentrated and dried under reduced pressure to obtain polyphenols from papaya seed meal.

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PUM

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Abstract

The invention relates to a method for extracting papaya seed meal polyphenol. The method comprises the following steps: (1) cleaning papaya seeds to remove impurities, drying and placing in a pulverizer to be pulverized, passing through a mesh sieve, and uniformly mixing to be ready for use; (2) adding an acetone extract to papaya seed meal powder, and placing in an ultrasonic cleaner for extracting; and (3) placing the extract in a rotary evaporator to be concentrated under reduced pressure, eluting with double distilled water to remove impurities, and then desorbing with ethanol until the eluent is colorless, concentrating under reduced pressure and drying to obtain papaya seed meal polyphenols. The polyphenols of papaya seed meal are extracted by ultrasonic-assisting, so that the great waste of the papaya seed meal is avoided and the comprehensive utilization value of papaya is improved. The method is simple to operate, low in cost, short in extraction time, high in extraction rate and high in purity, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of polyphenol extraction methods, in particular to a method for extracting polyphenols from papaya seed dregs. Background technique [0002] Polyphenols are phenolic secondary metabolites produced in plants and microorganisms, compounds with potential health-promoting effects, and are widely found in plant foods. Polyphenols have anti-oxidation function and prevent chronic diseases in the human body. According to research, polyphenols can also effectively prevent the derivatives produced by high-fat foods in the human body from adversely affecting the human body. [0003] Papaya is an important medicinal and edible plant in my country. It is rich in polyphenols and has physiological activities such as anti-oxidation, anti-influenza virus, anti-thrombosis, reducing ultraviolet damage, and inhibiting tumors. Papaya fruit is moderately sweet and sour, rich in nutrients such as vitamin C, various essential amin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/732A61P39/06
CPCA61K36/732A61K2236/331A61K2236/51
Inventor 宋鸟生
Owner 宋鸟生