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Sinorhizobium meliloti with potassium-releasing function and application of sinorhizobium meliloti

A technology of sweet-scented clover and Sinorhizobium, applied in the fields of application, potassium fertilizer, bacteria, etc., can solve the problems of effective potassium loss, damage to the soil ecological environment, and impact on agricultural production, so as to save costs, have broad application prospects, and shorten the production cycle Effect

Active Publication Date: 2015-04-01
河北绿茵生化科技有限公司
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AI Technical Summary

Problems solved by technology

[0002] Nitrogen, phosphorus, and potassium fertilizers are fertilizers that are in great demand in my country's agricultural production, especially potassium fertilizer resources, which are currently mainly imported in large quantities to meet domestic needs, but long-term use of chemical fertilizers will cause a large amount of effective potassium in the soil to be lost, which will damage the normal soil The ecological environment affects agricultural production. If the solidified slow-acting potassium can be turned into quick-acting potassium, this problem can be better solved, and the potassium-dissolving bacteria just have such a role

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Isolation and identification of strains

[0014] Use the selective medium to screen the strains capable of dissolving potassium from the soil samples, collect soil samples from Xingtai, Zhangjiakou and other places, choose Axubei medium and potassium bacteria selective medium as the screening medium, and pick the strains on the two plates Capsulated, smooth, transparent oil-drop-shaped colonies that can grow on any surface. The isolated bacterial strains were inoculated into potassium solution medium (sucrose 10g, Na 2 HPO 4 1g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 1g, FeCl 3 ·6H 2 O 0.005g, CaCO 3 0.1g, 1g potassium-rich shale, 18g agar, 1000ml distilled water, pH 7.0-7.2. ), cultivated for 15 days, and screened the strains with high content of soluble potassium in the fermentation broth by atomic absorption method, and finally screened high-efficiency potassium-solubilizing strain 32-2, identified the genus and species, and stored the strains in 30% glycer...

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PUM

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Abstract

The invention discloses sinorhizobium meliloti with a potassium-releasing function and an application of sinorhizobium meliloti. The sinorhizobium meliloti is a strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.8343 or is a direct passage culture of the strain. According to the strain, potassium-rich shale of the area can be dissociated into rapidly available potassium capable of being absorbed and utilized by plants, the production period of the strain can be greatly shortened, a large quantity of cost is saved, and the application prospect is very broad.

Description

technical field [0001] The invention relates to the fields of microbes and chemical fertilizers, in particular to a sweet-scented clover rhizobia with a potassium-decomposing effect and application thereof. Background technique [0002] Nitrogen, phosphorus, and potassium fertilizers are fertilizers that are in great demand in my country's agricultural production, especially potassium fertilizer resources, which are currently mainly imported in large quantities to meet domestic needs, but long-term use of chemical fertilizers will cause a large amount of effective potassium in the soil to be lost, which will damage the normal soil The ecological environment affects agricultural production. If the solidified slow-acting potassium can be turned into quick-acting potassium, this problem can be better solved, and the potassium-dissolving bacteria just have such a role. [0003] Due to the specific geographical location and geological composition of Yantong Mountain in Xuanhua Dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05D1/00C12R1/01
CPCC05D1/00C12N1/205C12R2001/01
Inventor 宋水山宋聪贾振华黄媛媛黄亚丽马宏赵芊
Owner 河北绿茵生化科技有限公司
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